<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/1" level="1" class="item" period="#period1">
<inline name="tocNum">Article 1</inline>
<inline name="tocHeading">Member States shall take all necessary steps to ensure that...</inline>
...</tocItem>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/2" level="1" class="item" period="#period1">
<inline name="tocNum">Article 2</inline>
<inline name="tocHeading">Member States shall bring into force the laws, regulations or...</inline>
...</tocItem>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/3" level="1" class="item" period="#period1">
<inline name="tocNum">Article 3</inline>
<inline name="tocHeading">This Directive is addressed to the Member States. </inline>
...</tocItem>
<tocItem href="" level="1" class="schedules" period="#period1"/>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/annex" level="2" class="schedule" period="#period1">
<inline name="tocNum">ANNEX</inline>
...</tocItem>
<inline name="tocHeading">IDENTIFICATION AND DETERMINATION OF GLYCEROL l-(4-AMINOBENZOATE) </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Solvent mixture: cyclohexane/propan-2-ol/stabilized dichloromethane 48/64/9 (v/v/v). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Development solvent: petroleum ether (40-60)/benzene/acetone/ammonium hydroxide solution (minimum 25 %...</inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Developing solution sodium nitrite: 1 g in 100 ml of...</inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Standard solutions: </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Silica gel 60 F254 plates, 0,25 mm thick, 200 mm...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal apparatus for thin layer chromatography. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Ultrasonic vibrator. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Millipore filter FH 0,5 μm or equivalent. </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Thin layer chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Development </inline>
<inline name="tocNum">5.3.1.</inline>
<inline name="tocHeading">Observe the plate under 254 nm UV light. </inline>
<inline name="tocNum">5.3.2.</inline>
<inline name="tocHeading">Spray the completely dried plate with the solution 3.3 (a)....</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate (KH2PO4). </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Zinc di(acetate) (Zn(CH3COO)2 · 2H2O). </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Acetic acid d 20 4 = 1,05 . </inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Tetrapotassium hexacyanoferrate, (K4(Fe(CN)6) · 3H2O). </inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Ethyl 4-hydroxybenzoate. </inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Alpha monoglyceryl 4-aminobenzoate. </inline>
<inline name="tocNum">4.8.</inline>
<inline name="tocHeading">Ethyl 4-aminobenzoate. </inline>
<inline name="tocNum">4.9.</inline>
<inline name="tocHeading">Phosphate buffer solution (0,02 M): dissolve 2,72 g of potassium...</inline>
<inline name="tocNum">4.10.</inline>
<inline name="tocHeading">Eluant: phosphate buffer solution (4.9)/methanol (4.1) 61/39(v/v) </inline>
<inline name="tocNum">4.11.</inline>
<inline name="tocHeading">Stock solution of alpha-monoglyceryl 4-aminobenzoate: weigh accurately about 40 mg...</inline>
<inline name="tocNum">4.12.</inline>
<inline name="tocHeading">Stock solution of ethyl 4-aminobenzoate: weigh accurately about 40 mg...</inline>
<inline name="tocNum">4.13.</inline>
<inline name="tocHeading">Internal standard solution: weigh accurately about 50 mg of ethyl...</inline>
<inline name="tocNum">4.14.</inline>
<inline name="tocHeading">Standard solutions: prepare four standard solutions by dissolving in 100...</inline>
<inline name="tocNum">4.15</inline>
<inline name="tocHeading">Carrez I solution: dissolve 26,5 g of tetrapotassium hexacyanoferrate (4.5)...</inline>
<inline name="tocNum">4.16.</inline>
<inline name="tocHeading">Carrez II solution: dissolve 54,9 g of zinc di(acetate) (4.3)...</inline>
<inline name="tocNum">4.17.</inline>
<inline name="tocHeading">Merck Lichrosorb RP-18, or equivalent, with an average particle size...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">The usual laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">High-performance chromatography equipment with a variable wavelength UV detector and...</inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Stainless-steel column: length: 250 mm; internal diameter: 4,6 mm; packing:...</inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">6.1.1.</inline>
<inline name="tocHeading">Weigh accurately about 1 g of sample into a 100...</inline>
<inline name="tocNum">6.1.2.</inline>
<inline name="tocHeading">Place the beaker in the ultrasonic bath (5.4) for 20...</inline>
<inline name="tocNum">6.1.3.</inline>
<inline name="tocHeading">With a pipette, transfer 3,0 ml of the filtrate obtained...</inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">6.2.1.</inline>
<inline name="tocHeading">Adjust the flow rate of the mobile phase (4.10) to...</inline>
<inline name="tocNum">6.2.2.</inline>
<inline name="tocHeading">Set the detector (5.2) to 274 nm. </inline>
<inline name="tocNum">6.2.3.</inline>
<inline name="tocHeading">With a microsyringe, inject at least two times 20 μl...</inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Calibration curve </inline>
<inline name="tocNum">6.3.1.</inline>
<inline name="tocHeading">Inject 20 μl of each of the standard solutions (4.14)...</inline>
<inline name="tocNum">6.3.2.</inline>
<inline name="tocHeading">For each concentration calculate the ratio between the peak areas...</inline>
<inline name="tocNum">6.3.3.</inline>
<inline name="tocHeading">Proceed in the same manner for ethyl 4-hydroxybenzoate. </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">From the calibration curve obtained in 6.3 read off the...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">From the mass ratios obtained in this way calculate the...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">8.1.</inline>
<inline name="tocHeading">For a 5 % (m/m) content of alpha-monoglyceryl 4-aminobenzoate, the...</inline>
<inline name="tocNum">8.2.</inline>
<inline name="tocHeading">For a 1 % (m/m) content of ethyl 4-aminobenzoate the...</inline>
<inline name="tocNum">9.</inline>
<inline name="tocHeading">NOTES </inline>
<inline name="tocNum">9.1.</inline>
<inline name="tocHeading">Before carrying out an analysis, check whether the sample contains...</inline>
<inline name="tocNum">9.2.</inline>
<inline name="tocHeading">In order to check the absence of any interference, repeat...</inline>
<inline name="tocHeading">DETERMINATION OF CHLOROBUTANOL </inline>
<inline name="tocHeading"/>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Chlorobutanol (1,1,1-trichloro-2-methylpropan-2-ol). </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">2,2,2-Trichloroethanol. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Absolute ethanol. </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Standard solution of chlorobutanol: 0,025 g in 100 ml ethanol...</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Standard solution of 2,2,2-trichloroethanol: 4 mg in 100 ml ethanol...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Gas chromatograph with electron detector, Ni 63. </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Preparation of sample </inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Gas chromatography conditions </inline>
<inline name="tocNum">6.2.1.</inline>
<inline name="tocHeading">The operating conditions must yield a resolution factor R ≥...</inline>
<inline name="tocNum">6.2.2.</inline>
<inline name="tocHeading">As examples, the following operating conditions provide the required resolution:...</inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Standard curve </inline>
<inline name="tocNum">6.4.</inline>
<inline name="tocHeading">Inject 1 μl of solution obtained in 6.1 and proceed...</inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">Calculate from the standard curve (6.3) the quantity ‘a’ expressed...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">The content of chlorobutanol in the sample is calculated according...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocHeading">Note </inline>
<inline name="tocHeading">IDENTIFICATION AND DETERMINATION OF QUININE </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Silica gel plates, without fluorescence indicators, 0,25 mm thick, 200...</inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Developing solvent: toluene /diethyl ether /dichloromethane /diethylamine /20/20/20/8 (v/v/v/v). </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Sulphuric acid (96 %; d 20 4 = 1,84 )....</inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Diethyl ether. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">Developing agent: carefully add 5 ml of sulphuric acid (3.4)...</inline>
<inline name="tocNum">3.7.</inline>
<inline name="tocHeading">Bromine. </inline>
<inline name="tocNum">3.8.</inline>
<inline name="tocHeading">Ammonium hydroxide solution (28 %; d 20 4 = 0,90...</inline>
<inline name="tocNum">3.9.</inline>
<inline name="tocHeading">Quinine, anhydrous. </inline>
<inline name="tocNum">3.10.</inline>
<inline name="tocHeading">Standard solution: weigh accurately about 100,0 mg of anhydrous quinine...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal equipment for thin layer chromatography. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Millipore filter, FH 0,5 ƒm or equivalent with suitable filtration...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Preparation of the sample </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Thin layer chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Development </inline>
<inline name="tocNum">5.3.1.</inline>
<inline name="tocHeading">Dry the plate at room temperature. </inline>
<inline name="tocNum">5.3.21.</inline>
<inline name="tocHeading">Spray with reagent 3.6. </inline>
<inline name="tocNum">5.3.3.</inline>
<inline name="tocHeading">Leave the plate to dry for one hour at room...</inline>
<inline name="tocNum">5.3.4.</inline>
<inline name="tocHeading">Observe under the light from a UV lamp adjusted to...</inline>
<inline name="tocNum">5.3.5.</inline>
<inline name="tocHeading">For further confirmation that quinine is present, the plate is...</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Acetonitrile. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate (KH2PO4). </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Orthophosphoric acid (85 %; d 20 4 = 1,7 )....</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Tetramethylaminium bromide. </inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Quinine, anhydrous. </inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Orthophosphoric acid solution (0,1 M): weigh 11,53 g of orthophosphoric...</inline>
<inline name="tocNum">4.8.</inline>
<inline name="tocHeading">Potassium dihyrogenorthophosphate solution (0,1 M): weigh 13,6 g of potassium...</inline>
<inline name="tocNum">4.9.</inline>
<inline name="tocHeading">Tetramethylammonium bromide solution: dissolve 15,40 g of tetramethylammonium bromide (4.4)...</inline>
<inline name="tocNum">4.10</inline>
<inline name="tocHeading">Eluant: orthophosphoric acid (4.7) /potassium dihydrogenorthophosphate (4.8) /tetramethylammonium bromide (4.9)/water/acetonitrile...</inline>
<inline name="tocNum">4.11.</inline>
<inline name="tocHeading">Silica treated with octadecylsilane, 10 μm. </inline>
<inline name="tocNum">4.12.</inline>
<inline name="tocHeading">Standard solutions: weigh accurately approximately 5,0, 10,0, 15,0 and 20,0...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Usual laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">High-performance liquid chromatography equipment with a variable wavelength detector. </inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Column: length: 250 mm; internal diameter: 4,6 mm; filling: silica...</inline>
<inline name="tocNum">5.5.</inline>
<inline name="tocHeading">Millipore filter FH 0,5 μm, or equivalent, with suitable filtration...</inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Calibration curve </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">From the calibration curve (6.3) determine the quantity in μg...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">The concentration of anhydrous quinine in the sample, as a...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF INORGANIC SULPHITES AND HYDROGEN SULPHITES</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Hydrochloric acid (4 M). </inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Potassium iodate starch paper or other suitable alternative. </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Flask (25 ml) fitted with a short reflux condenser. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Place about 2,5 g of sample in the flask (3.2)...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Mix and heat to boiling. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Test for the emission of sulphur dioxide either by smell...</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Hydrogen peroxide 0,2 % (m/v). Prepare daily. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Orthophosphoric acid ( d 25 4 = 1,75 ). </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Sodium hydroxide (0,01 M) standardized solution. </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Nitrogen. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">Indicator: mixture 1: 1 (v/v) of methyl red (0,03 %...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Distillation apparatus (see figure). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Weigh accurately about 2,5 g of sample into the distillation...</inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Add 60 ml of water and 50 ml of methanol...</inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Place 10 ml of hydrogen peroxide (3.1), 60 ml of...</inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Repeat 5.3 for the wash bottle E (see figure). </inline>
<inline name="tocNum">5.5.</inline>
<inline name="tocHeading">Assemble the apparatus and adjust the nitrogen (3.5) flow to...</inline>
<inline name="tocNum">5.6.</inline>
<inline name="tocHeading">Run 15 ml of orthophosphoric acid (3.2) from the funnel...</inline>
<inline name="tocNum">5.7.</inline>
<inline name="tocHeading">Heat rapidly to boiling and then simmer gently for a...</inline>
<inline name="tocNum">5.8.</inline>
<inline name="tocHeading">Detach the distillation receiver D. Rinse the tube and then...</inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">Sulphurdioxide distillation apparatus according to Tanner</inline>
<inline name="tocHeading">All dimensions in mm </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF CHLORATES OF THE ALKALI METALS</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Reference solutions: aqueous solutions of potassium chlorate, bromate and iodate...</inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Development solvent: ammonia solution (28% m/v) acetone/butanol (60/130/30 v/v/v). </inline>
<inline name="tocNum">2.3.</inline>
<inline name="tocHeading">Potassium iodide, aqueous solution (5 % m/v). </inline>
<inline name="tocNum">2.4.</inline>
<inline name="tocHeading">Starch solution (1 to 5 % m/v). </inline>
<inline name="tocNum">2.5.</inline>
<inline name="tocHeading">Hydrochloric acid (1 M). </inline>
<inline name="tocNum">2.6.</inline>
<inline name="tocHeading">Ready-for-use cellulose thin-layer plates (0,25 mm). </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Extract about 1 g of the sample with water, filter,...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Deposit 2 (μl on the plate (2.6) of the solution...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Place the plate in a tank and develop by ascending...</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Remove from the tank and allow the solvent to evaporate....</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Spray the plate with potassium iodide (2.3) and allow to...</inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Spray the plate with starch solution (2.4) and allow to...</inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Spray the plate with hydrochloric acid (2.5). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">EVALUATION </inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Acetic acid, 80 % (m/m). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Zinc powder. </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Silver nitrate standard solution (0,1 M). </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potentiometer equipped with a silver indicator electrode. </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Reduction of chlorate </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Determination of chloride </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF SODIUM IODATE</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Reference solutions. Aqueous solutions of potassium chlorate, bromate and iodate...</inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Development solvent. </inline>
<inline name="tocNum">2.3.</inline>
<inline name="tocHeading">Potassium iodide aqueous solution (5 % m/v). </inline>
<inline name="tocNum">2.4.</inline>
<inline name="tocHeading">Starch solution (1 to 5 % m/v). </inline>
<inline name="tocNum">2.5.</inline>
<inline name="tocHeading">Hydrochloric acid (1 M). </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Ready-for-use cellulose thin-layer chromatography (0,25 mm) plates. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Normal equipment for thin layer chromatography. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Extract about 1 g of the sample with water, filter,...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Deposit 2 µl of this solution onto the base line...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Place the plate in a tank and develop by ascending...</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Remove the plate from the tank and allow the solvent...</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Spray the plate with potassium iodide (2.3) and allow to...</inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Spray with starch (2.4) and allow to dry for about...</inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Finally spray with hydrochloric (2.5). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">EVALUATION </inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Hydrochloric acid (4 M). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Sodium sulphite aq, 5 % m/v. </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Sodium iodate stock solution. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate. </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Disodium hydrogenorthophosphate · 2H2O. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">HPLC mobile phase: dissolve 3,88 g potassium dihydrogenorthophosphate (3.4) and...</inline>
<inline name="tocNum">3.7.</inline>
<inline name="tocHeading">Universal indicator paper, pH 1-11. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Ordinary laboratory apparatus. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Circular filter paper, diameter 110 mm, Schleicher and Schüll No...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">High-performance liquid chromatograph with a variable wavelength detector. </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Columns: length: 120 mm; internal diameter: 4,6 mm; number: two...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.1.1.</inline>
<inline name="tocHeading">Fluid samples (shampoos) </inline>
<inline name="tocNum">5.1.2.</inline>
<inline name="tocHeading">Solid samples (soap) </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Calibration </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">CONFIRMATION </inline>
<inline name="tocNum">8.1.</inline>
<inline name="tocHeading">Principle </inline>
<inline name="tocNum">8.2.</inline>
<inline name="tocHeading">Procedure </inline>