OJ No L 262, 27. 9. 1976, p. 169.

OJ No L 224, 22. 8. 1985, p. 40.

OJ No L 383, 31. 12. 1980, p. 27.

OJ No L 185, 30. 6. 1982, p. 1.

OJ No L 291, 24. 10. 1983, p. 9.

http://www.legislation.gov.uk/eudr/1985/490/contentsFourth Commission Directive of 11 October 1985 on the approximation of the laws of the Member States relating to methods of analysis necessary for checking the composition of cosmetic products (85/490/EEC)Fourth Commission Directive of 11 October 1985 on the approximation of the laws of the Member States relating to methods of analysis necessary for checking the composition of cosmetic products (85/490/EEC)2021-02-11King's Printer of Acts of Parliamenthttps://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX:31985L0490texttext/xmlen1985-10-11Article 1Member States shall take all necessary steps to ensure that...Article 2Member States shall bring into force the laws, regulations or...Article 3This Directive is addressed to the Member States. ANNEXIDENTIFICATION AND DETERMINATION OF GLYCEROL l-(4-AMINOBENZOATE) A.IDENTIFICATION 1.SCOPE AND FIELD OF APPLICATION 2.PRINCIPLE 3.REAGENTS 3.1.Solvent mixture: cyclohexane/propan-2-ol/stabilized dichloromethane 48/64/9 (v/v/v). 3.2.Development solvent: petroleum ether (40-60)/benzene/acetone/ammonium hydroxide solution (minimum 25 %...3.3.Developing solution sodium nitrite: 1 g in 100 ml of...3.4.Standard solutions: 3.5.Silica gel 60 F254 plates, 0,25 mm thick, 200 mm...4.APPARATUS 4.1.Normal apparatus for thin layer chromatography. 4.2.Ultrasonic vibrator. 4.3.Millipore filter FH 0,5 μm or equivalent. 5.PROCEDURE 5.1.Sample preparation 5.2.Thin layer chromatography 5.3.Development 5.3.1.Observe the plate under 254 nm UV light. 5.3.2.Spray the completely dried plate with the solution 3.3 (a)....B.DETERMINATION 1.SCOPE AND FIELD OF APPLICATION 2.DEFINITION 3.PRINCIPLE 4.REAGENTS 4.1.Methanol. 4.2.Potassium dihydrogenorthophosphate (KH2PO4). 4.3.Zinc di(acetate) (Zn(CH3COO)2 · 2H2O). 4.4.Acetic acid d 20 4 = 1,05 . 4.5.Tetrapotassium hexacyanoferrate, (K4(Fe(CN)6) · 3H2O). 4.6.Ethyl 4-hydroxybenzoate. 4.7.Alpha monoglyceryl 4-aminobenzoate. 4.8.Ethyl 4-aminobenzoate. 4.9.Phosphate buffer solution (0,02 M): dissolve 2,72 g of potassium...4.10.Eluant: phosphate buffer solution (4.9)/methanol (4.1) 61/39(v/v) 4.11.Stock solution of alpha-monoglyceryl 4-aminobenzoate: weigh accurately about 40 mg...4.12.Stock solution of ethyl 4-aminobenzoate: weigh accurately about 40 mg...4.13.Internal standard solution: weigh accurately about 50 mg of ethyl...4.14.Standard solutions: prepare four standard solutions by dissolving in 100...4.15Carrez I solution: dissolve 26,5 g of tetrapotassium hexacyanoferrate (4.5)...4.16.Carrez II solution: dissolve 54,9 g of zinc di(acetate) (4.3)...4.17.Merck Lichrosorb RP-18, or equivalent, with an average particle size...5.APPARATUS 5.1.The usual laboratory equipment. 5.2.High-performance chromatography equipment with a variable wavelength UV detector and...5.3.Stainless-steel column: length: 250 mm; internal diameter: 4,6 mm; packing:...5.4.Ultrasonic bath. 6.PROCEDURE 6.1.Sample preparation 6.1.1.Weigh accurately about 1 g of sample into a 100...6.1.2.Place the beaker in the ultrasonic bath (5.4) for 20...6.1.3.With a pipette, transfer 3,0 ml of the filtrate obtained...6.2.Chromatography 6.2.1.Adjust the flow rate of the mobile phase (4.10) to...6.2.2.Set the detector (5.2) to 274 nm. 6.2.3.With a microsyringe, inject at least two times 20 μl...6.3.Calibration curve 6.3.1.Inject 20 μl of each of the standard solutions (4.14)...6.3.2.For each concentration calculate the ratio between the peak areas...6.3.3.Proceed in the same manner for ethyl 4-hydroxybenzoate. 7.CALCULATION 7.1.From the calibration curve obtained in 6.3 read off the...7.2.From the mass ratios obtained in this way calculate the...8.REPEATABILITY 8.1.For a 5 % (m/m) content of alpha-monoglyceryl 4-aminobenzoate, the...8.2.For a 1 % (m/m) content of ethyl 4-aminobenzoate the...9.NOTES 9.1.Before carrying out an analysis, check whether the sample contains...9.2.In order to check the absence of any interference, repeat...DETERMINATION OF CHLOROBUTANOL 1.SCOPE AND FIELD OF APPLICATION 2.DEFINITION 3.PRINCIPLE 4.REAGENTS 4.1.Chlorobutanol (1,1,1-trichloro-2-methylpropan-2-ol). 4.2.2,2,2-Trichloroethanol. 4.3.Absolute ethanol. 4.4.Standard solution of chlorobutanol: 0,025 g in 100 ml ethanol...4.5.Standard solution of 2,2,2-trichloroethanol: 4 mg in 100 ml ethanol...5.APPARATUS 5.1.Normal laboratory equipment. 5.2.Gas chromatograph with electron detector, Ni 63. 6.PROCEDURE 6.1.Preparation of sample 6.2.Gas chromatography conditions 6.2.1.The operating conditions must yield a resolution factor R ≥...6.2.2.As examples, the following operating conditions provide the required resolution:...6.3.Standard curve 6.4.Inject 1 μl of solution obtained in 6.1 and proceed...7.CALCULATION 7.1.Calculate from the standard curve (6.3) the quantity ‘a’ expressed...7.2.The content of chlorobutanol in the sample is calculated according...8.REPEATABILITY Note IDENTIFICATION AND DETERMINATION OF QUININE A.IDENTIFICATION 1.SCOPE AND FIELD OF APPLICATION 2.PRINCIPLE 3.REAGENTS 3.1.Silica gel plates, without fluorescence indicators, 0,25 mm thick, 200...3.2.Developing solvent: toluene /diethyl ether /dichloromethane /diethylamine /20/20/20/8 (v/v/v/v). 3.3.Methanol. 3.4.Sulphuric acid (96 %; d 20 4 = 1,84 )....3.5.Diethyl ether. 3.6.Developing agent: carefully add 5 ml of sulphuric acid (3.4)...3.7.Bromine. 3.8.Ammonium hydroxide solution (28 %; d 20 4 = 0,90...3.9.Quinine, anhydrous. 3.10.Standard solution: weigh accurately about 100,0 mg of anhydrous quinine...4.APPARATUS 4.1.Normal equipment for thin layer chromatography. 4.2.Ultrasonic bath. 4.3.Millipore filter, FH 0,5 ƒm or equivalent with suitable filtration...5.PROCEDURE 5.1.Preparation of the sample 5.2.Thin layer chromatography 5.3.Development 5.3.1.Dry the plate at room temperature. 5.3.21.Spray with reagent 3.6. 5.3.3.Leave the plate to dry for one hour at room...5.3.4.Observe under the light from a UV lamp adjusted to...5.3.5.For further confirmation that quinine is present, the plate is...B.DETERMINATION 1.SCOPE AND FIELD OF APPLICATION 2.DEFINITION 3.PRINCIPLE 4.REAGENTS 4.1.Acetonitrile. 4.2.Potassium dihydrogenorthophosphate (KH2PO4). 4.3.Orthophosphoric acid (85 %; d 20 4 = 1,7 )....4.4.Tetramethylaminium bromide. 4.5.Quinine, anhydrous. 4.6.Methanol. 4.7.Orthophosphoric acid solution (0,1 M): weigh 11,53 g of orthophosphoric...4.8.Potassium dihyrogenorthophosphate solution (0,1 M): weigh 13,6 g of potassium...4.9.Tetramethylammonium bromide solution: dissolve 15,40 g of tetramethylammonium bromide (4.4)...4.10Eluant: orthophosphoric acid (4.7) /potassium dihydrogenorthophosphate (4.8) /tetramethylammonium bromide (4.9)/water/acetonitrile...4.11.Silica treated with octadecylsilane, 10 μm. 4.12.Standard solutions: weigh accurately approximately 5,0, 10,0, 15,0 and 20,0...5.APPARATUS 5.1.Usual laboratory equipment. 5.2.Ultrasonic bath. 5.3.High-performance liquid chromatography equipment with a variable wavelength detector. 5.4.Column: length: 250 mm; internal diameter: 4,6 mm; filling: silica...5.5.Millipore filter FH 0,5 μm, or equivalent, with suitable filtration...6.PROCEDURE 6.1.Sample preparation 6.2.Chromatography 6.3.Calibration curve 7.CALCULATION 7.1.From the calibration curve (6.3) determine the quantity in μg...7.2.The concentration of anhydrous quinine in the sample, as a...8.REPEATABILITY IDENTIFICATION AND DETERMINATION OF INORGANIC SULPHITES AND HYDROGEN SULPHITESSCOPE AND FIELD OF APPLICATION A.IDENTIFICATION 1.PRINCIPLE 2.REAGENTS 2.1.Hydrochloric acid (4 M). 2.2.Potassium iodate starch paper or other suitable alternative. 3.APPARATUS 3.1.Normal laboratory equipment. 3.2.Flask (25 ml) fitted with a short reflux condenser. 4.PROCEDURE 4.1.Place about 2,5 g of sample in the flask (3.2)...4.2.Mix and heat to boiling. 4.3.Test for the emission of sulphur dioxide either by smell...B.DETERMINATION 1.DEFINITION 2.PRINCIPLE 3.REAGENTS 3.1.Hydrogen peroxide 0,2 % (m/v). Prepare daily. 3.2.Orthophosphoric acid ( d 25 4 = 1,75 ). 3.3.Methanol. 3.4.Sodium hydroxide (0,01 M) standardized solution. 3.5.Nitrogen. 3.6.Indicator: mixture 1: 1 (v/v) of methyl red (0,03 %...4.APPARATUS 4.1.Normal laboratory equipment. 4.2.Distillation apparatus (see figure). 5.PROCEDURE 5.1.Weigh accurately about 2,5 g of sample into the distillation...5.2.Add 60 ml of water and 50 ml of methanol...5.3.Place 10 ml of hydrogen peroxide (3.1), 60 ml of...5.4.Repeat 5.3 for the wash bottle E (see figure). 5.5.Assemble the apparatus and adjust the nitrogen (3.5) flow to...5.6.Run 15 ml of orthophosphoric acid (3.2) from the funnel...5.7.Heat rapidly to boiling and then simmer gently for a...5.8.Detach the distillation receiver D. Rinse the tube and then...6.CALCULATION 7.REPEATABILITY Sulphurdioxide distillation apparatus according to TannerAll dimensions in mm IDENTIFICATION AND DETERMINATION OF CHLORATES OF THE ALKALI METALSSCOPE AND FIELD OF APPLICATION A.IDENTIFICATION 1.PRINCIPLE 2.REAGENTS 2.1.Reference solutions: aqueous solutions of potassium chlorate, bromate and iodate...2.2.Development solvent: ammonia solution (28% m/v) acetone/butanol (60/130/30 v/v/v). 2.3.Potassium iodide, aqueous solution (5 % m/v). 2.4.Starch solution (1 to 5 % m/v). 2.5.Hydrochloric acid (1 M). 2.6.Ready-for-use cellulose thin-layer plates (0,25 mm). 3.APPARATUS 4.PROCEDURE 4.1.Extract about 1 g of the sample with water, filter,...4.2.Deposit 2 (μl on the plate (2.6) of the solution...4.3.Place the plate in a tank and develop by ascending...4.4.Remove from the tank and allow the solvent to evaporate....4.5.Spray the plate with potassium iodide (2.3) and allow to...4.6.Spray the plate with starch solution (2.4) and allow to...4.7.Spray the plate with hydrochloric acid (2.5). 5.EVALUATION B.DETERMINATION 1.DEFINITION 2.PRINCIPLE 3.REAGENTS 3.1.Acetic acid, 80 % (m/m). 3.2.Zinc powder. 3.3.Silver nitrate standard solution (0,1 M). 4.APPARATUS 4.1.Normal laboratory equipment. 4.2.Potentiometer equipped with a silver indicator electrode. 5.PROCEDURE 5.1.Sample preparation 5.2.Reduction of chlorate 5.3.Determination of chloride 6.CALCULATION 7.REPEATABILITY IDENTIFICATION AND DETERMINATION OF SODIUM IODATESCOPE AND FIELD OF APPLICATION A.IDENTIFICATION 1.PRINCIPLE 2.REAGENTS 2.1.Reference solutions. Aqueous solutions of potassium chlorate, bromate and iodate...2.2.Development solvent. 2.3.Potassium iodide aqueous solution (5 % m/v). 2.4.Starch solution (1 to 5 % m/v). 2.5.Hydrochloric acid (1 M). 3.APPARATUS 3.1.Ready-for-use cellulose thin-layer chromatography (0,25 mm) plates. 3.2.Normal equipment for thin layer chromatography. 4.PROCEDURE 4.1.Extract about 1 g of the sample with water, filter,...4.2.Deposit 2 µl of this solution onto the base line...4.3.Place the plate in a tank and develop by ascending...4.4.Remove the plate from the tank and allow the solvent...4.5.Spray the plate with potassium iodide (2.3) and allow to...4.6.Spray with starch (2.4) and allow to dry for about...4.7.Finally spray with hydrochloric (2.5). 5.EVALUATION B.DETERMINATION 1.DEFINITION 2.PRINCIPLE 3.REAGENTS 3.1.Hydrochloric acid (4 M). 3.2.Sodium sulphite aq, 5 % m/v. 3.3.Sodium iodate stock solution. 3.4.Potassium dihydrogenorthophosphate. 3.5.Disodium hydrogenorthophosphate · 2H2O. 3.6.HPLC mobile phase: dissolve 3,88 g potassium dihydrogenorthophosphate (3.4) and...3.7.Universal indicator paper, pH 1-11. 4.APPARATUS 4.1.Ordinary laboratory apparatus. 4.2.Circular filter paper, diameter 110 mm, Schleicher and Schüll No...4.3.High-performance liquid chromatograph with a variable wavelength detector. 4.4.Columns: length: 120 mm; internal diameter: 4,6 mm; number: two...5.PROCEDURE 5.1.Sample preparation 5.1.1.Fluid samples (shampoos) 5.1.2.Solid samples (soap) 5.2.Chromatography 5.3.Calibration 6.CALCULATION 7.REPEATABILITY 8.CONFIRMATION 8.1.Principle 8.2.Procedure

Fourth Commission Directive

of 11 October 1985

on the approximation of the laws of the Member States relating to methods of analysis necessary for checking the composition of cosmetic products

(85/490/EEC)

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Economic Community,

Having regard to Council Directive 76/768/EEC of 27 July 1976 on the approximation of the laws of the Member States relating to cosmetic products, as last amended by Directive 85/391/EEC, and in particular Article 8 (1) thereof,

Whereas Directive 76/768/EEC provides for the official testing of cosmetic products with the aim of ensuring that the conditions laid down by Community provisions concerning the composition of cosmetic products are satisfied;

Whereas all the necessary methods of analysis should be laid down as quickly as possible; whereas three steps towards the attainment of this objective having already been taken through the definition of certain methods in Commission Directive 80/1335/EEC, 82/434/EEC and 83/514/EEC, the fourth step is to consist in the definition of methods for the identification and determination of glycerol 1-(4-aminobenzoate), the determination of chlorobutanol, the identification and determination of quinine, the identification and determination of inorganic sulphites and hydrogen sulphites, the identification and determination of chlorates of the alkali metals and the identification and determination of sodium iodate;

Whereas the measures provided for in this Directive are in accordance with the opinion of the Committee on the Adaptation of Directive 76/768/EEC to Technical Progress,

HAS ADOPTED THIS DIRECTIVE:

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<notes source="#source">
<note class="footnote" eId="f00001">
<p>
<ref href="https://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.1976.262.01.0169.01.ENG">OJ No L 262, 27. 9. 1976, p. 169</ref>
.
</p>
</note>
<note class="footnote" eId="f00002">
<p>
<ref href="https://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.1985.224.01.0040.01.ENG">OJ No L 224, 22. 8. 1985, p. 40</ref>
.
</p>
</note>
<note class="footnote" eId="f00003">
<p>
<ref href="https://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.1980.383.01.0027.01.ENG">OJ No L 383, 31. 12. 1980, p. 27</ref>
.
</p>
</note>
<note class="footnote" eId="f00004">
<p>
<ref href="https://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.1982.185.01.0001.01.ENG">OJ No L 185, 30. 6. 1982, p. 1</ref>
.
</p>
</note>
<note class="footnote" eId="f00005">
<p>
<ref href="https://webarchive.nationalarchives.gov.uk/eu-exit/https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.1983.291.01.0009.01.ENG">OJ No L 291, 24. 10. 1983, p. 9</ref>
.
</p>
</note>
</notes>
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<ukm:Alternative URI="http://www.legislation.gov.uk/eudr/1985/490/pdfs/eudr_19850490_adopted_en.pdf" Date="1985-10-11" Size="1043158"/>
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<ukm:ScheduleParagraphs Value="262"/>
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<coverPage>
<toc>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/1" level="1" class="item" period="#period1">
<inline name="tocNum">Article 1</inline>
<inline name="tocHeading">Member States shall take all necessary steps to ensure that...</inline>
</tocItem>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/2" level="1" class="item" period="#period1">
<inline name="tocNum">Article 2</inline>
<inline name="tocHeading">Member States shall bring into force the laws, regulations or...</inline>
</tocItem>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/article/3" level="1" class="item" period="#period1">
<inline name="tocNum">Article 3</inline>
<inline name="tocHeading">This Directive is addressed to the Member States. </inline>
</tocItem>
<tocItem href="" level="1" class="schedules" period="#period1"/>
<tocItem href="http://www.legislation.gov.uk/eudr/1985/490/annex" level="2" class="schedule" period="#period1">
<inline name="tocNum">ANNEX</inline>
</tocItem>
<inline name="tocHeading">IDENTIFICATION AND DETERMINATION OF GLYCEROL l-(4-AMINOBENZOATE) </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Solvent mixture: cyclohexane/propan-2-ol/stabilized dichloromethane 48/64/9 (v/v/v). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Development solvent: petroleum ether (40-60)/benzene/acetone/ammonium hydroxide solution (minimum 25 %...</inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Developing solution sodium nitrite: 1 g in 100 ml of...</inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Standard solutions: </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Silica gel 60 F254 plates, 0,25 mm thick, 200 mm...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal apparatus for thin layer chromatography. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Ultrasonic vibrator. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Millipore filter FH 0,5 μm or equivalent. </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Thin layer chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Development </inline>
<inline name="tocNum">5.3.1.</inline>
<inline name="tocHeading">Observe the plate under 254 nm UV light. </inline>
<inline name="tocNum">5.3.2.</inline>
<inline name="tocHeading">Spray the completely dried plate with the solution 3.3 (a)....</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate (KH2PO4). </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Zinc di(acetate) (Zn(CH3COO)2 · 2H2O). </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Acetic acid d 20 4 = 1,05 . </inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Tetrapotassium hexacyanoferrate, (K4(Fe(CN)6) · 3H2O). </inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Ethyl 4-hydroxybenzoate. </inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Alpha monoglyceryl 4-aminobenzoate. </inline>
<inline name="tocNum">4.8.</inline>
<inline name="tocHeading">Ethyl 4-aminobenzoate. </inline>
<inline name="tocNum">4.9.</inline>
<inline name="tocHeading">Phosphate buffer solution (0,02 M): dissolve 2,72 g of potassium...</inline>
<inline name="tocNum">4.10.</inline>
<inline name="tocHeading">Eluant: phosphate buffer solution (4.9)/methanol (4.1) 61/39(v/v) </inline>
<inline name="tocNum">4.11.</inline>
<inline name="tocHeading">Stock solution of alpha-monoglyceryl 4-aminobenzoate: weigh accurately about 40 mg...</inline>
<inline name="tocNum">4.12.</inline>
<inline name="tocHeading">Stock solution of ethyl 4-aminobenzoate: weigh accurately about 40 mg...</inline>
<inline name="tocNum">4.13.</inline>
<inline name="tocHeading">Internal standard solution: weigh accurately about 50 mg of ethyl...</inline>
<inline name="tocNum">4.14.</inline>
<inline name="tocHeading">Standard solutions: prepare four standard solutions by dissolving in 100...</inline>
<inline name="tocNum">4.15</inline>
<inline name="tocHeading">Carrez I solution: dissolve 26,5 g of tetrapotassium hexacyanoferrate (4.5)...</inline>
<inline name="tocNum">4.16.</inline>
<inline name="tocHeading">Carrez II solution: dissolve 54,9 g of zinc di(acetate) (4.3)...</inline>
<inline name="tocNum">4.17.</inline>
<inline name="tocHeading">Merck Lichrosorb RP-18, or equivalent, with an average particle size...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">The usual laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">High-performance chromatography equipment with a variable wavelength UV detector and...</inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Stainless-steel column: length: 250 mm; internal diameter: 4,6 mm; packing:...</inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">6.1.1.</inline>
<inline name="tocHeading">Weigh accurately about 1 g of sample into a 100...</inline>
<inline name="tocNum">6.1.2.</inline>
<inline name="tocHeading">Place the beaker in the ultrasonic bath (5.4) for 20...</inline>
<inline name="tocNum">6.1.3.</inline>
<inline name="tocHeading">With a pipette, transfer 3,0 ml of the filtrate obtained...</inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">6.2.1.</inline>
<inline name="tocHeading">Adjust the flow rate of the mobile phase (4.10) to...</inline>
<inline name="tocNum">6.2.2.</inline>
<inline name="tocHeading">Set the detector (5.2) to 274 nm. </inline>
<inline name="tocNum">6.2.3.</inline>
<inline name="tocHeading">With a microsyringe, inject at least two times 20 μl...</inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Calibration curve </inline>
<inline name="tocNum">6.3.1.</inline>
<inline name="tocHeading">Inject 20 μl of each of the standard solutions (4.14)...</inline>
<inline name="tocNum">6.3.2.</inline>
<inline name="tocHeading">For each concentration calculate the ratio between the peak areas...</inline>
<inline name="tocNum">6.3.3.</inline>
<inline name="tocHeading">Proceed in the same manner for ethyl 4-hydroxybenzoate. </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">From the calibration curve obtained in 6.3 read off the...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">From the mass ratios obtained in this way calculate the...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">8.1.</inline>
<inline name="tocHeading">For a 5 % (m/m) content of alpha-monoglyceryl 4-aminobenzoate, the...</inline>
<inline name="tocNum">8.2.</inline>
<inline name="tocHeading">For a 1 % (m/m) content of ethyl 4-aminobenzoate the...</inline>
<inline name="tocNum">9.</inline>
<inline name="tocHeading">NOTES </inline>
<inline name="tocNum">9.1.</inline>
<inline name="tocHeading">Before carrying out an analysis, check whether the sample contains...</inline>
<inline name="tocNum">9.2.</inline>
<inline name="tocHeading">In order to check the absence of any interference, repeat...</inline>
<inline name="tocHeading">DETERMINATION OF CHLOROBUTANOL </inline>
<inline name="tocHeading"/>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Chlorobutanol (1,1,1-trichloro-2-methylpropan-2-ol). </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">2,2,2-Trichloroethanol. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Absolute ethanol. </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Standard solution of chlorobutanol: 0,025 g in 100 ml ethanol...</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Standard solution of 2,2,2-trichloroethanol: 4 mg in 100 ml ethanol...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Gas chromatograph with electron detector, Ni 63. </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Preparation of sample </inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Gas chromatography conditions </inline>
<inline name="tocNum">6.2.1.</inline>
<inline name="tocHeading">The operating conditions must yield a resolution factor R ≥...</inline>
<inline name="tocNum">6.2.2.</inline>
<inline name="tocHeading">As examples, the following operating conditions provide the required resolution:...</inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Standard curve </inline>
<inline name="tocNum">6.4.</inline>
<inline name="tocHeading">Inject 1 μl of solution obtained in 6.1 and proceed...</inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">Calculate from the standard curve (6.3) the quantity ‘a’ expressed...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">The content of chlorobutanol in the sample is calculated according...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocHeading">Note </inline>
<inline name="tocHeading">IDENTIFICATION AND DETERMINATION OF QUININE </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Silica gel plates, without fluorescence indicators, 0,25 mm thick, 200...</inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Developing solvent: toluene /diethyl ether /dichloromethane /diethylamine /20/20/20/8 (v/v/v/v). </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Sulphuric acid (96 %; d 20 4 = 1,84 )....</inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Diethyl ether. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">Developing agent: carefully add 5 ml of sulphuric acid (3.4)...</inline>
<inline name="tocNum">3.7.</inline>
<inline name="tocHeading">Bromine. </inline>
<inline name="tocNum">3.8.</inline>
<inline name="tocHeading">Ammonium hydroxide solution (28 %; d 20 4 = 0,90...</inline>
<inline name="tocNum">3.9.</inline>
<inline name="tocHeading">Quinine, anhydrous. </inline>
<inline name="tocNum">3.10.</inline>
<inline name="tocHeading">Standard solution: weigh accurately about 100,0 mg of anhydrous quinine...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal equipment for thin layer chromatography. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Millipore filter, FH 0,5 ƒm or equivalent with suitable filtration...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Preparation of the sample </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Thin layer chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Development </inline>
<inline name="tocNum">5.3.1.</inline>
<inline name="tocHeading">Dry the plate at room temperature. </inline>
<inline name="tocNum">5.3.21.</inline>
<inline name="tocHeading">Spray with reagent 3.6. </inline>
<inline name="tocNum">5.3.3.</inline>
<inline name="tocHeading">Leave the plate to dry for one hour at room...</inline>
<inline name="tocNum">5.3.4.</inline>
<inline name="tocHeading">Observe under the light from a UV lamp adjusted to...</inline>
<inline name="tocNum">5.3.5.</inline>
<inline name="tocHeading">For further confirmation that quinine is present, the plate is...</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Acetonitrile. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate (KH2PO4). </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Orthophosphoric acid (85 %; d 20 4 = 1,7 )....</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Tetramethylaminium bromide. </inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Quinine, anhydrous. </inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Orthophosphoric acid solution (0,1 M): weigh 11,53 g of orthophosphoric...</inline>
<inline name="tocNum">4.8.</inline>
<inline name="tocHeading">Potassium dihyrogenorthophosphate solution (0,1 M): weigh 13,6 g of potassium...</inline>
<inline name="tocNum">4.9.</inline>
<inline name="tocHeading">Tetramethylammonium bromide solution: dissolve 15,40 g of tetramethylammonium bromide (4.4)...</inline>
<inline name="tocNum">4.10</inline>
<inline name="tocHeading">Eluant: orthophosphoric acid (4.7) /potassium dihydrogenorthophosphate (4.8) /tetramethylammonium bromide (4.9)/water/acetonitrile...</inline>
<inline name="tocNum">4.11.</inline>
<inline name="tocHeading">Silica treated with octadecylsilane, 10 μm. </inline>
<inline name="tocNum">4.12.</inline>
<inline name="tocHeading">Standard solutions: weigh accurately approximately 5,0, 10,0, 15,0 and 20,0...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Usual laboratory equipment. </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Ultrasonic bath. </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">High-performance liquid chromatography equipment with a variable wavelength detector. </inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Column: length: 250 mm; internal diameter: 4,6 mm; filling: silica...</inline>
<inline name="tocNum">5.5.</inline>
<inline name="tocHeading">Millipore filter FH 0,5 μm, or equivalent, with suitable filtration...</inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">6.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">6.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">6.3.</inline>
<inline name="tocHeading">Calibration curve </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.1.</inline>
<inline name="tocHeading">From the calibration curve (6.3) determine the quantity in μg...</inline>
<inline name="tocNum">7.2.</inline>
<inline name="tocHeading">The concentration of anhydrous quinine in the sample, as a...</inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF INORGANIC SULPHITES AND HYDROGEN SULPHITES</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Hydrochloric acid (4 M). </inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Potassium iodate starch paper or other suitable alternative. </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Flask (25 ml) fitted with a short reflux condenser. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Place about 2,5 g of sample in the flask (3.2)...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Mix and heat to boiling. </inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Test for the emission of sulphur dioxide either by smell...</inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Hydrogen peroxide 0,2 % (m/v). Prepare daily. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Orthophosphoric acid ( d 25 4 = 1,75 ). </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Methanol. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Sodium hydroxide (0,01 M) standardized solution. </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Nitrogen. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">Indicator: mixture 1: 1 (v/v) of methyl red (0,03 %...</inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Distillation apparatus (see figure). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Weigh accurately about 2,5 g of sample into the distillation...</inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Add 60 ml of water and 50 ml of methanol...</inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Place 10 ml of hydrogen peroxide (3.1), 60 ml of...</inline>
<inline name="tocNum">5.4.</inline>
<inline name="tocHeading">Repeat 5.3 for the wash bottle E (see figure). </inline>
<inline name="tocNum">5.5.</inline>
<inline name="tocHeading">Assemble the apparatus and adjust the nitrogen (3.5) flow to...</inline>
<inline name="tocNum">5.6.</inline>
<inline name="tocHeading">Run 15 ml of orthophosphoric acid (3.2) from the funnel...</inline>
<inline name="tocNum">5.7.</inline>
<inline name="tocHeading">Heat rapidly to boiling and then simmer gently for a...</inline>
<inline name="tocNum">5.8.</inline>
<inline name="tocHeading">Detach the distillation receiver D. Rinse the tube and then...</inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">Sulphurdioxide distillation apparatus according to Tanner</inline>
<inline name="tocHeading">All dimensions in mm </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF CHLORATES OF THE ALKALI METALS</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Reference solutions: aqueous solutions of potassium chlorate, bromate and iodate...</inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Development solvent: ammonia solution (28% m/v) acetone/butanol (60/130/30 v/v/v). </inline>
<inline name="tocNum">2.3.</inline>
<inline name="tocHeading">Potassium iodide, aqueous solution (5 % m/v). </inline>
<inline name="tocNum">2.4.</inline>
<inline name="tocHeading">Starch solution (1 to 5 % m/v). </inline>
<inline name="tocNum">2.5.</inline>
<inline name="tocHeading">Hydrochloric acid (1 M). </inline>
<inline name="tocNum">2.6.</inline>
<inline name="tocHeading">Ready-for-use cellulose thin-layer plates (0,25 mm). </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Extract about 1 g of the sample with water, filter,...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Deposit 2 (μl on the plate (2.6) of the solution...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Place the plate in a tank and develop by ascending...</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Remove from the tank and allow the solvent to evaporate....</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Spray the plate with potassium iodide (2.3) and allow to...</inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Spray the plate with starch solution (2.4) and allow to...</inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Spray the plate with hydrochloric acid (2.5). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">EVALUATION </inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Acetic acid, 80 % (m/m). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Zinc powder. </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Silver nitrate standard solution (0,1 M). </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Normal laboratory equipment. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Potentiometer equipped with a silver indicator electrode. </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Reduction of chlorate </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Determination of chloride </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">IDENTIFICATION AND DETERMINATION OF SODIUM IODATE</inline>
<inline name="tocHeading">SCOPE AND FIELD OF APPLICATION </inline>
<inline name="tocNum">A.</inline>
<inline name="tocHeading">IDENTIFICATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">2.1.</inline>
<inline name="tocHeading">Reference solutions. Aqueous solutions of potassium chlorate, bromate and iodate...</inline>
<inline name="tocNum">2.2.</inline>
<inline name="tocHeading">Development solvent. </inline>
<inline name="tocNum">2.3.</inline>
<inline name="tocHeading">Potassium iodide aqueous solution (5 % m/v). </inline>
<inline name="tocNum">2.4.</inline>
<inline name="tocHeading">Starch solution (1 to 5 % m/v). </inline>
<inline name="tocNum">2.5.</inline>
<inline name="tocHeading">Hydrochloric acid (1 M). </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Ready-for-use cellulose thin-layer chromatography (0,25 mm) plates. </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Normal equipment for thin layer chromatography. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Extract about 1 g of the sample with water, filter,...</inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Deposit 2 µl of this solution onto the base line...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">Place the plate in a tank and develop by ascending...</inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Remove the plate from the tank and allow the solvent...</inline>
<inline name="tocNum">4.5.</inline>
<inline name="tocHeading">Spray the plate with potassium iodide (2.3) and allow to...</inline>
<inline name="tocNum">4.6.</inline>
<inline name="tocHeading">Spray with starch (2.4) and allow to dry for about...</inline>
<inline name="tocNum">4.7.</inline>
<inline name="tocHeading">Finally spray with hydrochloric (2.5). </inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">EVALUATION </inline>
<inline name="tocNum">B.</inline>
<inline name="tocHeading">DETERMINATION </inline>
<inline name="tocNum">1.</inline>
<inline name="tocHeading">DEFINITION </inline>
<inline name="tocNum">2.</inline>
<inline name="tocHeading">PRINCIPLE </inline>
<inline name="tocNum">3.</inline>
<inline name="tocHeading">REAGENTS </inline>
<inline name="tocNum">3.1.</inline>
<inline name="tocHeading">Hydrochloric acid (4 M). </inline>
<inline name="tocNum">3.2.</inline>
<inline name="tocHeading">Sodium sulphite aq, 5 % m/v. </inline>
<inline name="tocNum">3.3.</inline>
<inline name="tocHeading">Sodium iodate stock solution. </inline>
<inline name="tocNum">3.4.</inline>
<inline name="tocHeading">Potassium dihydrogenorthophosphate. </inline>
<inline name="tocNum">3.5.</inline>
<inline name="tocHeading">Disodium hydrogenorthophosphate · 2H2O. </inline>
<inline name="tocNum">3.6.</inline>
<inline name="tocHeading">HPLC mobile phase: dissolve 3,88 g potassium dihydrogenorthophosphate (3.4) and...</inline>
<inline name="tocNum">3.7.</inline>
<inline name="tocHeading">Universal indicator paper, pH 1-11. </inline>
<inline name="tocNum">4.</inline>
<inline name="tocHeading">APPARATUS </inline>
<inline name="tocNum">4.1.</inline>
<inline name="tocHeading">Ordinary laboratory apparatus. </inline>
<inline name="tocNum">4.2.</inline>
<inline name="tocHeading">Circular filter paper, diameter 110 mm, Schleicher and Schüll No...</inline>
<inline name="tocNum">4.3.</inline>
<inline name="tocHeading">High-performance liquid chromatograph with a variable wavelength detector. </inline>
<inline name="tocNum">4.4.</inline>
<inline name="tocHeading">Columns: length: 120 mm; internal diameter: 4,6 mm; number: two...</inline>
<inline name="tocNum">5.</inline>
<inline name="tocHeading">PROCEDURE </inline>
<inline name="tocNum">5.1.</inline>
<inline name="tocHeading">Sample preparation </inline>
<inline name="tocNum">5.1.1.</inline>
<inline name="tocHeading">Fluid samples (shampoos) </inline>
<inline name="tocNum">5.1.2.</inline>
<inline name="tocHeading">Solid samples (soap) </inline>
<inline name="tocNum">5.2.</inline>
<inline name="tocHeading">Chromatography </inline>
<inline name="tocNum">5.3.</inline>
<inline name="tocHeading">Calibration </inline>
<inline name="tocNum">6.</inline>
<inline name="tocHeading">CALCULATION </inline>
<inline name="tocNum">7.</inline>
<inline name="tocHeading">REPEATABILITY </inline>
<inline name="tocNum">8.</inline>
<inline name="tocHeading">CONFIRMATION </inline>
<inline name="tocNum">8.1.</inline>
<inline name="tocHeading">Principle </inline>
<inline name="tocNum">8.2.</inline>
<inline name="tocHeading">Procedure </inline>
</toc>
</coverPage>
<preface>
<longTitle>
<p>
<inline name="uppercase">Fourth Commission Directive</inline>
</p>
<p>of 11 October 1985</p>
<p>on the approximation of the laws of the Member States relating to methods of analysis necessary for checking the composition of cosmetic products</p>
<p>(85/490/EEC)</p>
</longTitle>
</preface>
<preamble>
<p>THE COMMISSION OF THE EUROPEAN COMMUNITIES,</p>
<p>Having regard to the Treaty establishing the European Economic Community,</p>
<p>
Having regard to Council Directive 76/768/EEC of 27 July 1976 on the approximation of the laws of the Member States relating to cosmetic products
<noteRef href="#f00001" class="footnote" marker="1"/>
, as last amended by Directive 85/391/EEC
<noteRef href="#f00002" class="footnote" marker="2"/>
, and in particular Article 8 (1) thereof,
</p>
<p>Whereas Directive 76/768/EEC provides for the official testing of cosmetic products with the aim of ensuring that the conditions laid down by Community provisions concerning the composition of cosmetic products are satisfied;</p>
<p>
Whereas all the necessary methods of analysis should be laid down as quickly as possible; whereas three steps towards the attainment of this objective having already been taken through the definition of certain methods in Commission Directive 80/1335/EEC
<noteRef href="#f00003" class="footnote" marker="3"/>
, 82/434/EEC
<noteRef href="#f00004" class="footnote" marker="4"/>
and 83/514/EEC
<noteRef href="#f00005" class="footnote" marker="5"/>
, the fourth step is to consist in the definition of methods for the identification and determination of glycerol 1-(4-aminobenzoate), the determination of chlorobutanol, the identification and determination of quinine, the identification and determination of inorganic sulphites and hydrogen sulphites, the identification and determination of chlorates of the alkali metals and the identification and determination of sodium iodate;
</p>
<p>Whereas the measures provided for in this Directive are in accordance with the opinion of the Committee on the Adaptation of Directive 76/768/EEC to Technical Progress,</p>
<p>HAS ADOPTED THIS DIRECTIVE:</p>
</preamble>
<body/>
</act>
</akomaNtoso>