Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot (c. 85)

[F1 ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Textual Amendments

F1 Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.

Appendix 3 Buffers for test procedures

2. Buffers for the IF test U.K.

2.1. IF-Buffer (10 mM phosphate buffered saline (PBS), pH 7,2) U.K.

This buffer is used for dilution of antibodies.

Na 2 HPO 4 .12H 2 O	2,7 g
NaH 2 PO 4 .2H 2 O	0,4 g
NaCl	8,0 g
Distilled water	1.0 L

Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.

2.2. IF-buffer-Tween U.K.

This buffer is used to wash slides.

Add 0,1 % Tween 20 to the IF buffer.

2.3. Phosphate buffered glycerol, pH 7,6 U.K.

This buffer is used as a mountant fluid on the windows of IF slides to enhance fluorescence.

Na 2 HPO 4 .12H 2 O	3,2 g
NaH 2 PO 4 .2H 2 O	0,15 g
Glycerol	50 ml
Distilled water	100 ml

Anti-fading mountant solutions are commercially available e.g. Vectashield ^® (Vector Laboratories) or Citifluor ^® (Leica).]