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[F1ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Appendix 5 Media for isolation and culture of C. m. subsp. sepedonicus

(a) General growth media U.K.

Yeast peptone glucose agar (YPGA) U.K.
Yeast Extract (Difco) 5,0 g
Bacto-Peptone (Difco) 5,0 g
D(+) Glucose (monohydrate) 10,0 g
Bacto-Agar (Difco) 15,0 g
Distilled water 1,0 L

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.]