Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot (c. 85)

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[F1 ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Textual Amendments

F1 Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.

Appendix 9 Gram stain procedure (Hucker's modification) (Doetsch, 1981) (1)

Staining procedure U.K.

1.

Prepare smears, air dry and heat fix.

2.

Flood slide with crystal violet solution for one minute.

3.

Wash briefly with tap water.

4.

Flood with Lugol's iodine for one minute.

5.

Wash with tap water and blot dry.

6.

Decolourise with 95 % ethanol, added dropwise, until no further colour is removed or immerse with gentle agitation for 30 seconds.

7.

Wash in tap water and blot dry.

8.

Flood with safranin solution for 10 seconds.

9.

Wash with tap water and blot dry.

Gram positive bacteria stain violet-blue; Gram negative bacteria stain pink-red.]

(1)

[F1 Commercially available solutions and staining kits can also be used.]

Textual Amendments

F1 Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.