[F1Staining procedure U.K.
1.
Prepare smears, air dry and heat fix.
2.
Flood slide with crystal violet solution for one minute.
3.
Wash briefly with tap water.
4.
Flood with Lugol's iodine for one minute.
5.
Wash with tap water and blot dry.
6.
Decolourise with 95 % ethanol, added dropwise, until no further colour is removed or immerse with gentle agitation for 30 seconds.
7.
Wash in tap water and blot dry.
8.
Flood with safranin solution for 10 seconds.
9.
Wash with tap water and blot dry.
Gram positive bacteria stain violet-blue; Gram negative bacteria stain pink-red.]
Textual Amendments
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[F1Commercially available solutions and staining kits can also be used.]
Textual Amendments