Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al. (c. 57)

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[F1 ANNEX II U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.

Textual Amendments

F1 Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..

Appendix 2 Media for isolation and culture of R. solanacearum

(a) General growth media U.K.

Nutrient Agar (NA) U.K.

Nutrient Agar (Difco)	23,0 g
Distilled water	1,0 L

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.

Yeast Peptone Glucose Agar (YPGA) U.K.

Yeast extract (Difco)	5,0 g
Bacto-Peptone (Difco)	5,0 g
D(+) Glucose (monohydrate)	10,0 g
Bacto-Agar (Difco)	15,0 g
Distilled water	1,0 L

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.

Sucrose Peptone Agar (SPA) U.K.

Sucrose	20,0 g
Bacto-Peptone (Difco)	5,0 g
K 2 HPO 4	0,5 g
MgSO 4 .7H 2 O	0,25 g
Bacto-Agar (Difco)	15,0 g
Distilled water	1,0 l
pH 7,2 – 7,4

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.

Kelman’s Tetrazolium Medium U.K.

Casamino acids (Difco)	1,0 g
Bacto-Peptone (Difco)	10,0 g
Dextrose	5,0 g
Bacto-Agar (Difco)	15,0 g
Distilled water	1,0 l

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.

Cool to 50 °C and add a filter-sterilised solution of 2,3,5-triphenyl tetrazolium chloride (Sigma) to obtain a final concentration of 50 mg per l.]