[F1(a) General growth media U.K.
Nutrient Agar (NA) U.K.
Nutrient Agar (Difco) | 23,0 g |
Distilled water | 1,0 L |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.
Yeast Peptone Glucose Agar (YPGA) U.K.
Yeast extract (Difco) | 5,0 g |
Bacto-Peptone (Difco) | 5,0 g |
D(+) Glucose (monohydrate) | 10,0 g |
Bacto-Agar (Difco) | 15,0 g |
Distilled water | 1,0 L |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.
Sucrose Peptone Agar (SPA) U.K.
Sucrose | 20,0 g |
Bacto-Peptone (Difco) | 5,0 g |
K 2 HPO 4 | 0,5 g |
MgSO 4 .7H 2 O | 0,25 g |
Bacto-Agar (Difco) | 15,0 g |
Distilled water | 1,0 l |
pH 7,2 – 7,4 |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.
Kelman’s Tetrazolium Medium U.K.
Casamino acids (Difco) | 1,0 g |
Bacto-Peptone (Difco) | 10,0 g |
Dextrose | 5,0 g |
Bacto-Agar (Difco) | 15,0 g |
Distilled water | 1,0 l |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.
Cool to 50 °C and add a filter-sterilised solution of 2,3,5-triphenyl tetrazolium chloride (Sigma) to obtain a final concentration of 50 mg per l.]