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Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.
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The following bacterial isolates are recommended for use as standard reference material either as positive controls (Table 1) or during optimisation of tests to avoid cross-reactions (Table 2). All strains are commercially available from:
National Collection of Plant Pathogenic Bacteria (NCPPB), Central Science Laboratory, York, UK
Culture Collection of the Plant Protection Service (PD), Wageningen, the Netherlands.
Collection Française de Bactéries Phytopathogènes (CFBP), INRA Station Phytobactériologie, Angers, France.
a Use as standard reference strain of R. solanacearum biovar 2 (race 3). | ||||
NCPPB code | SMT # | Other codes | Country of origin | Biovar |
---|---|---|---|---|
NCPPB 4153 | 6 | CFBP 4582, Pr 3020, EURS11 | Egypt | 2 |
NCPPB 4154 | 10 | CFBP 4585, 550, EURS21 | Turkey | 2 |
NCPPB 3857 | 12 | CFBP 4587, Pr 1140, EURS26 | England | 2 |
NCPPB 1584 | 23 | CFBP 4598, EURS49 | Cyprus | 2 |
NCPPB 2505 | 24 | CFBP 4599, EURS50 | Sweden | 2 |
NCPPB 4155 | 26 | CFBP 4601, 502, EURS55 | Belgium | 2 |
NCPPB 4156 a | 71 a | PD 2762, CFBP 3857 | Netherlands | 2 |
NCPPB 4157 | 66 | LNPV 15.59 | France | 2 |
NCPPB 4158 | 39 | CFBP 4608, Port 448, EURS80 | Portugal | 2 |
NCPPB 4160 | 69 | IVIA-1632-2 | Spain | 2 |
NCPPB 4161 | 76 | B3B | Germany | 2 |
NCPPB 325 | 41 | CFBP 2047, KEL60-1, R842 | USA | 1 |
NCPPB 3967 | 42 | CFBP 4610, R285, GONg7 | Costa Rica | 1 |
NCPPB 4028 | 43 | CFBP 4611, R303/571, CIP310, SEQ205 | Colombia | 2 |
NCPPB 3985 | 44 | CFBP 4612, R578, CIP312 | Peru | 2T |
NCPPB 3989 | 45 | CFBP 4613, R568, CIP226 | Brazil | 2T |
NCPPB 3996 | 46 | CFBP 3928, R276/355, CIP72, SEQ225 | Peru | 3 |
NCPPB 3997 | 47 | CFBP 4614, R280/363, CIP49, HAY0131a | Australia | 3 |
NCPPB 4029 | 48 | CFBP 4615, R297/349, CIP121, CMIb2861 | Sri Lanka | 4 |
NCPPB 4005 | 49 | CFBP 4616, R470 | Philippines | 4 |
NCPPB 4011 | 50 | CFBP 4617, R288, HEmps2 | China | 5 |
Note: Authenticity of the above strains can be guaranteed only if obtained from an authentic culture collection. U.K.
a Potential cross-reacting strain in serological tests (IF and/or ELISA) with polyclonal antisera. | |||
b Strain from which PCR product can be amplified in some laboratories of a similar size to that expected using specific primers OLI-1 and Y-2 (see Appendix 6). | |||
c Likely to cross-react in most tests but known to occur only on banana in Indonesia. | |||
NCPPB code | SMT # | Other code | Identification |
---|---|---|---|
NCPPB 4162 | 51 | CFBP 1954 | Bacillus polymyxa a |
NCPPB 4163 | 52 | CFBP 1538 | Pseudomonas marginalis pv. marginalis a |
NCPPB 4164 | — | CFBP 2227 | Burkholderia cepacia b |
NCPPB 4165 | — | CFBP 2459 | Ralstonia pickettii b |
NCPPB 4166 | 58 | CFBP 3567 CSL Pr1150 | Ralstonia pickettii a |
NCPPB 4167 | 60 | CFBP 4618 PD 2778 | Ralstonia sp. a |
NCPPB 1127 | 53 | CFBP 3575 | Burkholderia andropogonis a |
NCPPB 353 | 54 | CFBP 3572 | Burkholderia caryophylli a |
NCPPB 945 | 55 | CFBP 3569 | Burkholderia cepacia a |
NCPPB 3708 | 56 | CFBP 3574 | Burkholderia glumae a |
NCPPB 3590 | 57 | CFBP 3573 | Burkholderia plantarii a |
NCPPB 3726 | 59 | CFBP 3568 | Banana Blood Disease Bacterium a b c |
NCPPB 4168 | 61 | CFBP 4619 IPO S339 | Enterobacter sp. a |
NCPPB 4169 | 62 | IPO 1695 | Enterobacter sp. a |
NCPPB 4170 | 63 | CFBP 4621 IPO S306 | Ochrobactrum anthropi a b |
NCPPB 4171 | 64 | CFBP 4622 IPO 1693 | Curtobacterium sp. a b |
NCPPB 4172 | 65 | IPO 1696a | Pseudomonas sp. a |
NCPPB 4173 | — | PD 2318 | Aureobacterium sp. b |
NCPPB 4174 | 81 | IVIA 1844.06 | Flavobacterium sp. a b |
The following standard control material is available from the NCPPB culture collection.
Freeze dried pellet of potato extract from 200 healthy potato tubers as negative control for all tests.
Freeze dried pellet of potato extract from 200 healthy potato tubers containing 10 3 to 10 4 and 10 4 to 10 6 cells R. solanacearum biovar 2 (strain NCPPB 4156 = PD 2762 = CFBP 3857) as positive controls for serological and PCR tests. Since cell viability is affected during freeze-drying, these are not suitable as standard controls for isolation or bioassay tests.
Formalin-fixed suspensions of R. solanacearum biovar 2 (strain NCPPB 4156 = PD 2762 = CFBP 3857) at 10 6 cells per ml as positive controls for serological tests.]
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