Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al. (c. 57)

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[F1 ANNEX II U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.

Textual Amendments

F1 Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..

Appendix 4 Buffers for test procedures

2. Buffers for the IF test U.K.

2.1. IF-Buffer (10 mM phosphate buffered saline (PBS), pH 7.2) U.K.

This buffer is used for dilution of antibodies

Na 2 HPO 4 .12H 2 O	2,7 g
NaH 2 PO 4 .2H 2 O	0,4 g
NaCL	8,0 g
Distilled water	1,0 l

Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 minutes.

2.2. IF-buffer-Tween U.K.

This buffer is used to wash slides.

Add 0,1 % Tween 20 to the IF buffer.

2.3. Phosphate buffered glycerol, pH 7,6 U.K.

This buffer is used as a mountant fluid on the windows of IF slides to enhance fluorescence.

Na 2 HPO 4 .12H 2 O	3,2 g
NaH 2 PO 4 .2H 2 O	0,15 g
Glycerol	50 ml
Distilled water	100 ml

Anti-fading mountant solutions are commercially available e.g. Vectashield ^® (Vector Laboratories) or Citifluor ^® (Leica).]