1.
[F1Count the mean number of typical fluorescent cells per field of view (c).
2.
Calculate the number of typical fluorescent cells per microscope slide window (C).
C = c × S/s
| where | S | = | surface area of window of multispot slide |
| and | s | = | surface area of objective field |
| s = πi 2 /4G 2 K 2 | where | i | = | field coefficient (varies from 8 to 24 depending upon ocular type) |
| K | = | tube coefficient (1 or 1,25) | ||
| G | = | magnification of objective (100 ×, 40 × etc.). |
3.
Calculate the number of typical fluorescent cells per ml of re-suspended pellet (N).
N = C × 1 000 /y × F
| where | y | = | volume of re-suspended pellet on each window |
| and | F | = | dilution factor of re-suspended pellet.] |