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Textual Amendments
The testing procedure is intended for potato tubers and plants with symptoms typical or suspect of brown rot or vascular wilt. It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on (selective) medium and, in case of a positive result, identification of the culture as Ralstonia solanacearum .
The testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests ( 3 ), based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as R. solanacearum A positive result from only one of the screening tests is not sufficient to consider the sample suspect.
Screening tests and isolation tests must permit detection of 10 3 to 10 4 cells/ml of resuspended pellet, included as positive controls in each series of tests.