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ANNEX I

E 315 ERYTHORBIC ACID

SynonymsIsoascorbic acid
D-Araboascorbic acid
Definition
Chemical nameD-Erythro-hex-2-enoic acid γ-lactone
Isoascorbic acid
D-Isoascorbic acid
Einecs201-928-0
Chemical formulaC6H8O6
Molecular weight176,13
AssayContent not less than 98 % on the anhydrous basis
DescriptionWhite to slightly yellow crystalline solid which darkens gradually on exposure to light
Identification
A.Melting range
About 164 oC to 172 oC with decomposition
B.Positive test for ascorbic acid/colour reaction
Purity
Loss on dryingNot more than 0,4 % after drying under reduced pressure on silica gel for 3 hours
Sulphated ashNot more than 0,3 %
Specific rotation[α]10 % (w/v) aqueous solution between -16,5o to -18,0o
OxalateTo a solution of 1 g in 10 ml of water add 2 drops of glacial acetic acid and 5 ml of 10 % calcium acetate solution. The solution should remain clear
LeadNot more than 2 mg/kg