Directive 2009/54/EC of the European Parliament and of the CouncilDangos y teitl llawn

1.3. Criteria for microbiological analyses at source

These analyses shall include:

1.3.1.

a demonstration of the absence of parasites and pathogenic micro-organisms;

1.3.2.

a quantitative determination of the revivable colony count indicative of faecal contamination:

(a)

absence of Escherichia coli and other coliforms in 250 ml at 37 °C and 44,5 °C;

(b)

absence of faecal streptococci in 250 ml;

(c)

absence of sporulated sulphite-reducing anaerobes in 50 ml;

(d)

absence of Pseudomonas aeruginosa in 250 ml;

1.3.3.

determination of the revivable total colony count per ml of water:

(a)

at 20 to 22 °C in 72 hours on agar-agar or an agar-gelatine mixture;

(b)

at 37 °C in 24 hours on agar-agar.