Textual Amendments
The usual laboratory equipment and in particular the following:
250 ml flask fitted with a reflux condenser with ground-glass joints.
500 ml separating funnel.
250 ml flasks.
Complete apparatus for analysis by thin-layer chromatography using 20 x 20 cm glass plates.
Ultraviolet lamp with a wavelength of 254 or 366 nm.
100 μl and 500 μl microsyringes.
Cylindrical filter funnel with a G3 porous septum (porosity 15-40 μm) of diameter approximately 2 cm and a depth of 5 cm, suitable for filtration under vacuum with male ground-glass joint.
50 ml vacuum conical flask with ground-glass female joint, which can be fitted to the filter funnel (point 3.7).
10 ml test tube with a tapering bottom and a sealing glass stopper.
Gas chromatograph suitable for use with a capillary column with split injection system, consisting of:
A thermostatic chamber for columns capable of maintaining the desired temperature with an accuracy of ± 1°C;
A temperature-adjustable injection unit with a persilanised glass vaporising element and split system;
A flame ionisation detector (FID);
Data acquisition system suitable for use with the FID detector (point 3.10.3.), capable of manual integration.
Fused-silica capillary column of length 20 to 30 m, internal diameter 0,25 to 0,32 mm, coated with 5 % diphenyl - 95 % dimethylpolysiloxane (SE-52 or SE-54 stationary phase or equivalent), to a uniform thickness between 0,10 and 0,30 μm.
Microsyringe, of 10 ml capacity, for gas chromatography, with cemented needle suitable for split injection.
Calcium dichloride desiccator]