Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (c. 2568)

[F1 ANNEX XVIII U.K. DETERMINATION OF THE DIFFERENCE BETWEEN ACTUAL AND THEORETICAL CONTENT OF TRIACYLGLYCEROLS WITH ECN 42

Textual Amendments

F1 Substituted by Commission Implementing Regulation (EU) No 299/2013 of 26 March 2013 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.

6. EXAMPLE (THE NUMBERS REFER TO THE SECTIONS IN THE TEXT OF THE METHOD) U.K.

— 4.5.1. Calculation of moles % fatty acids from GLC data (normalised area %) U.K.

The following data are obtained for the fatty acid composition by GLC:

FA	P	S	Po	O	L	Ln
MW	256,4	284,5	254,4	282,5	280,4	278,4
Area %	10,0	3,0	1,0	75,0	10,0	1,0

— 4.5.3 Conversion of area % into moles for all fatty acids (see formula (1)) U.K.

moles P

=

moles S

=

moles Po

=

moles O

=

moles L

=

moles Ln

=

Total

=

0,35821 moles TAGs

— 4.5.4 Normalisation of fatty acid moles to 100 % (see formula (2)) U.K.

moles % P(1,2,3)

=

moles % S(1,2,3)

=

moles % Po(1,2,3)

=

moles % O(1,2,3)

=

moles % L(1,2,3)

=

moles % Ln(1,2,3)

=

Total moles %

=

100 %

Sum of the saturated and unsaturated fatty acids in the 1,2,3-position of TAGs (see formula (3)):

— 4.5.5 Calculation of the fatty acid composition in 2- and 1,3-positions of the TAGs U.K.

— 4.5.5.1 Saturated fatty acids in 2-position [P(2) and S(2)] (see formula (4)) U.K.

— 4.5.5.2 Unsaturated fatty acids in 2-position [Po(1,3), O(1,3), L(1,3) and Ln(1,3)] (see formula (5)) U.K.

— 4.5.5.3 Fatty acids in 1,3-positions [P(1,3), S(1,3), Po(1,3), O(1,3), L(1,3) and Ln(1,3)] (see formula (6)) U.K.

— 4.5.6. Calculation of triacylglycerols U.K.

From the calculated fatty acid composition in sn-2- and sn-1,3-positions:

FA in	1,3-pos	2-pos
P	16,004 %	0,653 %
S	4,325 %	0,177 %
Po	1,015 %	1,262 %
O	68,526 %	85,296 %
L	9,204 %	11,457 %
Ln	0,927 %	1,153 %
Sum	100,0 %	100,0 %

the following triacylglycerols are calculated:

• LLL

• PoPoPo

• PoLL with 1 positional isomer

• SLnLn with 1 positional isomer

• PoPoL with 1 positional isomer

• PPoLn with 2 positional isomers

• OLLn with 2 positional isomers

• PLLn with 2 positional isomers

• PoOLn with 2 positional isomers

— 4.5.6.1. TAGs with one fatty acid (LLL, PoPoPo) (see formula (7)) U.K.

, = 0,09706 mol LLL

— 4.5.6.2 TAGs with two fatty acids (PoLL, SLnLn, PoPoL) (see formula (8)) U.K.

0,03210 mol PoLL

0,00094 mol SLnLn

0,00354 mol PoPoL

— 4.5.6.3 TAGs with three different fatty acids (PoPLn, OLLn, PLLn, PoOLn) See formula (9) U.K.

0,00761 mol PPoLn

0,43655 mol OLLn

0,06907 mol PLLn

0,04812 mol PoOLn

ECN42 = 0,69512 mol TAGs]