[F16. EXAMPLE (THE NUMBERS REFER TO THE SECTIONS IN THE TEXT OF THE METHOD) U.K.
— 4.5.1. Calculation of moles % fatty acids from GLC data (normalised area %) U.K.
The following data are obtained for the fatty acid composition by GLC:
| FA | P | S | Po | O | L | Ln |
|---|---|---|---|---|---|---|
| MW | 256,4 | 284,5 | 254,4 | 282,5 | 280,4 | 278,4 |
| Area % | 10,0 | 3,0 | 1,0 | 75,0 | 10,0 | 1,0 |
— 4.5.3 Conversion of area % into moles for all fatty acids (see formula (1)) U.K.
Total
=
0,35821 moles TAGs
— 4.5.4 Normalisation of fatty acid moles to 100 % (see formula (2)) U.K.
Total moles %
=
100 %
Sum of the saturated and unsaturated fatty acids in the 1,2,3-position of TAGs (see formula (3)):
— 4.5.5 Calculation of the fatty acid composition in 2- and 1,3-positions of the TAGs U.K.
— 4.5.5.1 Saturated fatty acids in 2-position [P(2) and S(2)] (see formula (4)) U.K.
— 4.5.5.2 Unsaturated fatty acids in 2-position [Po(1,3), O(1,3), L(1,3) and Ln(1,3)] (see formula (5)) U.K.
— 4.5.5.3 Fatty acids in 1,3-positions [P(1,3), S(1,3), Po(1,3), O(1,3), L(1,3) and Ln(1,3)] (see formula (6)) U.K.
— 4.5.6. Calculation of triacylglycerols U.K.
From the calculated fatty acid composition in sn-2- and sn-1,3-positions:
| FA in | 1,3-pos | 2-pos |
|---|---|---|
| P | 16,004 % | 0,653 % |
| S | 4,325 % | 0,177 % |
| Po | 1,015 % | 1,262 % |
| O | 68,526 % | 85,296 % |
| L | 9,204 % | 11,457 % |
| Ln | 0,927 % | 1,153 % |
| Sum | 100,0 % | 100,0 % |
the following triacylglycerols are calculated:
LLL
PoPoPo
PoLL with 1 positional isomer
SLnLn with 1 positional isomer
PoPoL with 1 positional isomer
PPoLn with 2 positional isomers
OLLn with 2 positional isomers
PLLn with 2 positional isomers
PoOLn with 2 positional isomers
— 4.5.6.1. TAGs with one fatty acid (LLL, PoPoPo) (see formula (7)) U.K.
— 4.5.6.2 TAGs with two fatty acids (PoLL, SLnLn, PoPoL) (see formula (8)) U.K.
0,03210 mol PoLL
0,00094 mol SLnLn
0,00354 mol PoPoL
— 4.5.6.3 TAGs with three different fatty acids (PoPLn, OLLn, PLLn, PoOLn) See formula (9) U.K.
0,00761 mol PPoLn
0,43655 mol OLLn
0,06907 mol PLLn
0,04812 mol PoOLn
ECN42 = 0,69512 mol TAGs]