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Commission Regulation (EC) No 1622/2000 (repealed)Dangos y teitl llawn
Commission Regulation (EC) No 1622/2000 of 24 July 2000 laying down certain detailed rules for implementing Regulation (EC) No 1493/1999 on the common organisation of the market in wine and establishing a Community code of oenological practices and processes (repealed)
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Changes over time for: ANNEX IX
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ANNEX IXU.K.Determination of the loss of organic matter from ion exchange resins(Article 12 of this Regulation)
1.SCOPE AND AREA OF APPLICATIONU.K.
The method determines the loss of organic matter from ion exchange resins.
2.DEFINITIONU.K.
The loss of organic matter from ion exchange resins. The loss of organic matter is determined by the method specified.
3.PRINCIPLEU.K.
Extracting solvents are passed through prepared resins and the weight of organic matter extracted is determined gravimetrically.
4.REAGENTSU.K.
All reagents should be of analytical quality.
Extracting solvents.
4.1.Distilled water or de-ionised water of equivalent purity.U.K.
4.2.Ethanol, 15 % v/v. Prepare by mixing 15 parts of absolute ethanol with 85 parts of water (4. 1 l).U.K.
4.3.Acetic acid, 5 % m/m. Prepare by mixing 5 parts of glacial acetic acid with 95 parts of water (4.1).U.K.
5.APPARATUSU.K.
5.1.Ion exchange chromatography columns.U.K.
5.2.Measuring cylinders, capacity 2 l.U.K.
5.3.Evaporating dishes capable of withstanding a muffle furnace at 850 °C.U.K.
5.4.Drying oven, thermostatically controlled at 105 ± 2 °C.U.K.
5.5.Muffle furnace, thermostatically controlled at 850 ± 25 °C.U.K.
5.6.Analytical balance, accurate to 0,1 mg.U.K.
5.7.Evaporator, hot plate or infra-red evaporator.U.K.
6.PROCEDUREU.K.
6.1.Add to each of three separate ion exchange chromatography columns (5.1) 50 ml of the ion exchange resin to be tested, washed and treated in accordance with the manufacturer's directions for preparing resins for use with food.U.K.
6.2.For the anionic resins, pass the three extracting solvents (4.1, 4.2 and 4.3) separately through the prepared columns (6.1) at a flow rate of 350 to 450 ml/h. Discard the first litre of eluate in each case and collect the next two litres in measuring cylinders (5.2). For the cationic resins, pass only solvents 4.1 and 4.2 through the columns prepared for this purpose.U.K.
6.3.Evaporate the three eluates over a hot plate or with an infra-red evaporator (5.7) in separate evaporating dishes (5.3) which have been previously cleaned and weighed (m0). Place the dishes in an oven (5.4) and dry to constant weight (m1).U.K.
6.4.After recording the constant weight (6.3), place the evaporating dish in the muffle furnace (5.5) and ash to constant weight (m2).U.K.
6.5.Calculate the organic matter extracted (7.1). If the result is greater than 1 mg/l, carry out a blank test on the reagents and recalculate the weight of organic matter extracted.U.K.
The blank test should be carried out by repeating sections 6.3 and 6.4 but using two litres of the extracting solvent, to give weights m3 and m4 in sections 6.3 and 6.4 respectively.
7.EXPRESSION OF RESULTSU.K.
7.1.Formula and calculation of resultsU.K.
The organic matter extracted from ion exchange resins, in mg/l, is given by:
500 (m1 – m2)
where m1 and m2 are expressed in grams.
The corrected weight (mg/l) of the organic matter extracted from ion exchange resins is given by:
500 (m1 – m2 – m3 + m4)
where m1, m2, m3 and m4 are expressed in grams.
7.2.The difference in the results between two parallel determinations carried out on the same sample must not exceed 0.2 mg/l.U.K.
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