Commission Regulation (EC) No 423/2008 of 8 May 2008 on laying down certain detailed rules for implementing Council Regulation (EC) No 1493/1999 and establishing a Community code of oenological practices and processes (Codified version) (repealed) (c. 423)

ANNEX VI

Requirements for beta-glucanase (Article 10 of this Regulation)

1. International code for beta-glucanase: E.C. 3-2-1-58

2. Beta-glucan hydrolase (breaking down the glucan in Botrytis cinerea)

3. Origin: Trichoderma harzianum

4. Area of application: breaking down the beta-glucans present in wines, in particular those produced from botrytised grapes

5. Maximum dose: 3 g of the enzymatic preparation containing 25 % total organic solids (TOS) per hectolitre

6. Chemical and microbiological purity specifications:

Loss on drying:	Less than 10 %
Heavy metals:	Less than 30 ppm
Pb:	Less than 10 ppm
As:	Less than 3 ppm
Total coliforms:	Absent
Escherichia coli	Absent in 25 g sample
Salmonella spp:	Absent in 25 g sample
Aerobic count:	Less than 5 × 104 cells/g