3.LABORATORY ANALYSESU.K.
3.1.Preparation of the samplesU.K.
At the laboratory samples shall be kept refrigerated until examination, which shall be started within 48 hours following receipt and within 96 hours after sampling.
The pair(s) of boot/sock swabs shall be carefully unpacked to avoid dislodging adherent faecal material, pooled and placed in 225 ml buffered peptone water (BPW) which has been pre-warmed to room temperature. The boot/sock swabs shall be fully immersed in BPW and therefore more BPW may be added if necessary.
The dust sample shall preferably be analysed separately. However for fattening flocks, the competent authority may decide to allow it to be pooled with the pair of boot/sock swabs for analysis.
The sample shall be swirled to fully saturate it and culture shall be continued by using the detection method set out in point 3.2.
Other samples (e.g. from breeding flocks or hatcheries) shall be prepared in accordance with point 2.2.2 of the Annex to Regulation (EU) No 200/2010.
F1...
Textual Amendments
F1Words in Annex point 3.1 omitted (31.12.2020) by virtue of The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 10(6)(f); 2020 c. 1, Sch. 5 para. 1(1)
[F23.2. Detection method U.K.
The detection of Salmonella must be carried out according to ISO 6579-1, as it has effect for the time being.]
Textual Amendments
F2Annex point 3.2 substituted (31.12.2020) by The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 10(6)(g); 2020 c. 1, Sch. 5 para. 1(1)
3.3.SerotypingU.K.
For breeding flocks of turkeys, at least one isolate from each positive sample shall be serotyped, following the White-Kauffmann-Le Minor scheme.
For fattening flocks of turkeys, at least one isolate from each positive sample taken by the competent authority shall be serotyped, following the White-Kauffmann-Le Minor scheme.
Food business operators shall at least ensure that for all isolates none of them belong to the serotypes Salmonella Enteritidis or Salmonella Typhimurium including monophasic strains with the antigenic formula [X11 ,4,[5],12:i:-].
Editorial Information
X1Substituted by Corrigendum to Commission Regulation (EU) No 1190/2012 of 12 December 2012 concerning a Union target for the reduction of Salmonella Enteritidis and Salmonella Typhimurium in flocks of turkeys, as provided for in Regulation (EC) No 2160/2003 of the European Parliament and of the Council (Official Journal of the European Union L 340 of 13 December 2012).
[F33.4. Alternative methods U.K.
Alternative methods may be used instead of the methods for detection and serotyping provided for in points 3.1, 3.2 and 3.3 of this Annex, if validated [F4in accordance with] [F5EN ISO 16140-2 (for alternative detection methods) or EN ISO 16140-6 (for validation of the confirmation or typing procedure of the typing method)].]
Textual Amendments
F4Words in Annex point 3.4 substituted (31.12.2020) by The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 10(6)(h); 2020 c. 1, Sch. 5 para. 1(1)
F5Words in Annex substituted (4.8.2023) by The Validating Alternative Methods for Salmonella Typing (Amendment) Regulations 2023 (S.I. 2023/793), regs. 1(1), 2(2)
Textual Amendments
3.5.Storage of strainsU.K.
Laboratories shall ensure that at least one isolated strain of Salmonella spp. per flock and per year can be collected by the competent authority and stored for possible future phage typing or anti-microbial susceptibility testing, using the normal methods for culture collection, which must ensure integrity of the strains for a minimum of two years from the date of analysis.
The competent authority may decide that isolates of Salmonella spp. from sampling by food business operators shall also be stored for future phagetyping or antimicrobial susceptibility testing to provide for isolates to be tested in accordance with [F6Commission Implementing Decision 2013/652/EU] .
Textual Amendments
F6Words in Annex point 3.5 substituted (31.12.2020) by The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 10(6)(i); 2020 c. 1, Sch. 5 para. 1(1)