- Y Diweddaraf sydd Ar Gael (Diwygiedig)
- Pwynt Penodol mewn Amser (17/07/2014)
- Gwreiddiol (Fel y’i mabwysiadwyd gan yr UE)
Commission Implementing Regulation (EU) No 809/2014 of 17 July 2014 laying down rules for the application of Regulation (EU) No 1306/2013 of the European Parliament and of the Council with regard to the integrated administration and control system, rural development measures and cross compliance
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Point in time view as at 17/07/2014.
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This method seeks to determine the Δ9-tetrahydrocannabinol (hereinafter referred to as THC) content of varieties of hemp (Cannabis sativa L.) As appropriate, the method involves applying procedure A or B herein described.
The method is based on the quantitative determination of Δ9-THC by gas chromatography (GC) after extraction with a suitable solvent.
Procedure A shall be used for checks on production as provided for in Article 32(6) of Regulation (EU) No 1307/2013 and Article 30(g) of this Regulation.
Procedure B shall be used in cases as referred to in Article 32(6) of Regulation (EU) No 1307/2013 and Article 36(6) of this Regulation.
Member States may authorise sampling to be carried out during the period from the start of flowering to 20 days after the start of flowering provided that, for each variety grown, other representative samples are taken in accordance with the first subparagraph during the period from 20 days after the start of flowering to 10 days after the end of flowering.
Procedure A: the sample shall comprise parts of 50 plants per field.
Procedure B: the sample shall comprise parts of 200 plants per field.
Each sample shall be placed in a fabric or paper bag, without crushing it, and be sent to the laboratory for analysis.
The Member State may provide for a second sample to be collected for counteranalysis, if required, to be kept either by the producer or by the body responsible for the analysis.
Drying of the samples shall begin as soon as possible and, in any case, within 48 hours using any method below 70 °C.
Samples shall be dried to a constant weight and to a moisture content of between 8 % and 13 %.
After drying, the samples shall be stored without crushing them at below 25 °C in a dark place.
Stems and seeds over 2 mm in size shall be removed from the dried samples.
The dried samples shall be grinded to obtain a semi-fine powder (passing through a 1 mm mesh sieve).
The powder may be stored for 10 weeks at below 25 °C in a dark, dry place.
Δ9-tetrahydrocannabinol, pure for chromatographic purposes,
Squalane, pure for chromatographic purposes, as an internal standard.
35 mg of squalane per 100 ml hexane.
100 mg of the powdered test sample shall be weighed, be placed in a centrifuge tube and 5 ml of extraction solution shall be added containing the internal standard.
The sample shall be placed in an ultrasound bath and be left for 20 minutes. It shall be centrifuged for five minutes at 3 000 r.p.m. and then the supernatant THC solution shall be removed. The solution shall be injected into the chromatograph and a quantitative analysis shall be carried out.
gas chromatograph with a flame ionisation detector and a split/splitless injector,
column allowing good separation of cannabinoids, for example a glass capillary column 25 m long and 0,22 mm in diameter impregnated with a 5 % non-polar phenyl-methyl-siloxane phase.
At least three points for procedure A and five points for procedure B, including points 0.04 and 0.50 mg/ml Δ9-THC in extraction solution.
The following conditions are given as an example for the column referred to in (a):
oven temperature 260 °C
injector temperature 300 °C
detector temperature 300 °C
The findings shall be expressed to two decimal places in grams of Δ9-THC per 100 grams of analytical sample dried to constant weight. A tolerance of 0,03 g per 100 g shall apply.
Procedure A: one determination per test sample.
However, where the result obtained is above the limit laid down in Article 32(6) of Regulation (EU) No 1307/2013, a second determination shall be carried out per analysis sample and the mean value of the two determinations shall be taken as the result.
Procedure B: the result shall correspond to the mean value of two determinations per test sample.
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