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Commission Implementing Regulation (EU) 2019/805Dangos y teitl llawn

Commission Implementing Regulation (EU) 2019/805 of 17 May 2019 concerning the authorisation of a preparation of muramidase produced by Trichoderma reesei DSM 32338 as a feed additive for chickens for fattening and minor poultry species for fattening (holder of authorisation DSM Nutritional Products Ltd, represented in EU by DSM Nutritional Products Sp. Z o.o) (Text with EEA relevance)

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ANNEX

a

One LSU(F) unit is defined as the amount of enzyme that increases the fluorescence of 12,5 μg/ml fluorescein-labelled peptidoglycan per minute at pH 6,0 and 30 °C by a value that corresponds to the fluorescence of approximately 0,06 nmol fluorescein isothiocyanate isomer I.

b

Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity /kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: other zootechnical additives (improvement of the feed to gain ratio)
4d16DSM Nutritional Products Ltd represented in the EU by DSM Nutritional Products Sp. Z o.o

Muramidase

(EC 3.2.1.17)

Additive composition

Preparation of muramidase (EC 3.2.1.17) (lysozyme) produced by Trichoderma reesei (DSM 32338) having a minimum activity of 60 000 LSU(F)/ga

Solid and liquid forms

Characterisation of the active substance

Muramidase (EC 3.2.1.17) (lysozyme) produced by Trichoderma reesei (DSM 32338)

Method of analysis b

For the quantification of muramidase:

fluorescence-based enzyme assay method that determines the enzyme-catalysed depolymerisation of a fluorescein-labelled peptidoglycan preparation at pH 6,0 and 30 °C.

Chickens for fattening

Minor poultry species for fattening

25 000 LSU(F)

1.In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated.

2.For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment including skin and breathing protections.

9 June 2029

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