regulations 3, 4 and 7
| No in Annex 1 to 79/923/EEC | Parameter | Units | Requirements to be satisfied | Reference methods of analysis | Minimum sampling and measuring frequency |
|---|---|---|---|---|---|
| 1. | pH | pH unit | ≥7 and ≤9 | — electrometry Measured in situ at the time of sampling | Quarterly |
| 3. | Coloration (after filtration) | mg Pt/1 | A discharge affecting shell- fish waters must not cause the colour of the waters after filtration to deviate by more than 10 mg Pt/1 from the colour of waters not so affected. | — Filter through a 0.45 um membrane Photometric method, using the platinum/cobalt scale | Quarterly |
| 4. | Suspended solids | mg/1 | A discharge affecting shell- fish waters must not cause the suspended solids content of the water to exceed by more than 30% the content of waters not so affected | Filtration through a 0.45 μm membrane, drying at 105°C and weighing — Centrifuging (for at least 5 minutes, with mean acceleration 2,800 to 3,200g), drying at 105°C and weighing | Quarterly |
| 5. | Salinity | ‰ | ≤40‰ — A discharge affecting shellfish waters must not cause their salinity to exceed by more than 10% the salinity of waters not so affected | Conductivity | Monthly |
| 6. | Dissolved oxygen | saturation % | ≥70% (average value) Should an individual measurement indicate a value lower than 70%, measurements shall be repeated An individual measurement may not indicate a value of less than 60% unless there are no harmful consequences for the development of shellfish colonies | — Winkler’s method — Electrochemical method | Monthly, with a minimum of 1 sample represent- ative of low oxygen conditions on the day of sampling. However, where major daily variations are suspected, a minimum of 2 samples in 1 day shall be taken |
| 7. | Petroleum hydrocarbons | Hydrocarbons must not be present in the shellfish water in such quantities as to:
| Visual examination | Quarterly | |
| 8. | Organohalo- genated substances | The concentration of each substance in the shellfish waters or in shellfish flesh must not reach or exceed a level which has harmful effects on the shellfish and their larvae | Gas chromatography after extraction with suitable solvents and purification | Half-yearly | |
| 9. | Metals
| mg/1 | The concentration of each substance in the shellfish waters or in the shellfish flesh must not exceed a level which gives rise to harmful effects on the shellfish and their larvae The synergic effects of these metals must be taken into consideration | Spectrometry of atomic absorption preceded, where appropriate, by concentration and/or extraction | Half-yearly |
| 11. | Substances affecting the taste of shellfish | Concentration should be lower than that which is liable to impair the taste of the shellfish | Examination of the shellfish by tasting where the presence of one of these substances is presumed |
regulations 5, 6 and 7
| No in Annex 1 to 79/923/EEC | Parameter | Units | Guideline values and comments | Reference methods of analysis | Minimum sampling and measuring frequency |
|---|---|---|---|---|---|
| 2. | Temperature | °C | A discharge affecting shellfish waters must not cause the temperature of the water to exceed by more than 2°C the temperature of waters not so affected | — Thermometry Measured in situ at the time of sampling | Quarterly |
| 5. | Salinity | ‰ | 12 to 38‰ | Conductivity | Monthly |
| 6. | Dissolved oxygen | saturation % | ≥80% |
| Monthly, with a minimum of 1 sample representative of low oxygen conditions on the day of sampling. However, where major daily variations are suspected, a minimum of 2 samples in 1 day shall be taken |
| 8. | Organohalo- genated substances | The concentration of each substance in shellfish flesh must be so limited that in contributes, in accordance with Article 1 of Directive 79/923/EEC, to the high quality of shellfish products | Gas chromatography after extraction with suitable solvents and purification | Half-yearly | |
| 9. | Metals
| mg/1 | The concentration of each substance in shellfish flesh must be so limited that it contributes in accordance with Article 1 of Directive 79/923/EEC, to the high quality of shellfish products | Spectrometry of atomic absorption preceded, where appropriate, by concentration and/or extraction | Half-yearly |
| 10. | Faecal coliforms | ≤300 in the shellfish flesh and intervalvular liquid | Method of dilution with fermentation in liquid substrates in at least 3 tubes in 3 dilutions. Subculturing of the positive tubes on a confirmation medium. Count according to MPN (most probable number). Incubation temperature 44° ± 0.5°C | Quarterly |