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The Water Supply (Water Quality) Regulations 2010
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Regulation 16
SCHEDULE 4ANALYTICAL METHODOLOGY
Regulation 16
Table A1Parameters for which, Subject to Regulation 16(7), Methods of Analysis are Prescribed
| (1) | (2) |
|---|---|
| Parameter | Method |
| Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. |
| Coliform bacteria | ISO 9308-1 |
| Colony count 22°C-enumeration of culturable microorganisms | prEN ISO 6222 |
| Colony count 37°C-enumeration of culturable microorganisms | prEN ISO 6222 |
| Enterococci | ISO 7899-2 |
| Escherichia coli (E coli) | ISO 9308-1 |
| *The composition of m-CP agar is: | |
| Basal medium | |
| Tryptose | 30.0g |
| Yeast extract | 20.0g |
| Sucrose | 5.0g |
| L-cysteine hydrochloride | 1.0g |
| MgSO4·7H2O | 0.1g |
| Bromocresol purple | 40.0mg |
| Agar | 15.0g |
| Water | 1,000.0ml |
| Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add: | |
| D-cycloserine | 400.0mg |
| Polymyxine-B sulphate | 25.0mg |
| Indoxyl-[beta]-D-glucoside to be dissolved in 8 ml sterile water before addition | 60.0mg |
| Filter-sterilised 0.5% phenolphthalein diphosphate solution | 20.0 ml |
| Filter-sterilised 4.5% FeCl3·6H2O | 2.0 ml |
Table A2Parameters for which, Subject to Regulation 16(7), Methods of Analysis are Prescribed
| (1) | (2) | (3) | (4) |
|---|---|---|---|
| Parameters | Trueness % of prescribed concentration or value or specification | Precision % of prescribed concentration or value or specification | Limit of detection % of prescribed concentration or value or specification |
| Notes: | |||
a The method of analysis should determine total cyanide in all forms. | |||
b The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. | |||
c The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1. | |||
d The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1. | |||
e The performance characteristics apply to the prescribed value of 4NTU. | |||
f The performance characteristics apply to the specification of 1NTU for water leaving treatment works. | |||
| Aluminium | 10 | 10 | 10 |
| Ammonium | 10 | 10 | 10 |
| Antimony | 25 | 25 | 25 |
| Arsenic | 10 | 10 | 10 |
| Benzene | 25 | 25 | 25 |
| Benzo(a)pyrene | 25 | 25 | 25 |
| Boron | 10 | 10 | 10 |
| Bromate | 25 | 25 | 25 |
| Cadmium | 10 | 10 | 10 |
| Chloride | 10 | 10 | 10 |
| Chromium | 10 | 10 | 10 |
| Colour | 10 | 10 | 10 |
| Conductivity | 10 | 10 | 10 |
| Copper | 10 | 10 | 10 |
| Cyanide a | 10 | 10 | 10 |
| 1,2-dichloroethane | 25 | 25 | 10 |
| Fluoride | 10 | 10 | 10 |
| Iron | 10 | 10 | 10 |
| Lead | 10 | 10 | 10 |
| Manganese | 10 | 10 | 10 |
| Mercury | 20 | 10 | 20 |
| Nickel | 10 | 10 | 10 |
| Nitrate | 10 | 10 | 10 |
| Nitrite | 10 | 10 | 10 |
| Pesticides and related products b | 25 | 25 | 25 |
| Polycyclic aromatic hydrocarbons c | 25 | 25 | 25 |
| Selenium | 10 | 10 | 10 |
| Sodium | 10 | 10 | 10 |
| Sulphate | 10 | 10 | 10 |
| Tetrachloromethane | 20 | 20 | 20 |
| Trichloroethene d | 25 | 25 | 10 |
| Trihalomethanes: Total c | 25 | 25 | 10 |
| Turbidity e | 10 | 10 | 10 |
| Turbidity f | 25 | 25 | 25 |
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