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2000/428/EC: Commission DecisionShow full title

2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance)

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C.The polymerase chain reaction (PCR) for genome detectionU.K.

1.Nucleic acid recognition methods can be used to detect swine vesicular disease viral genome in clinical material using the PCR and to establish relationships between isolates of swine vesicular disease virus by determining the nucleotide sequence of part of the genome. Techniques using the PCR have been developed to improve the sensitivity of diagnosis. Slightly different reverse transcriptase-PCR procedures have been described using primers corresponding to highly conserved regions in the 1C and 1D genes.U.K.
2.The PCR technique is rapid (the results are usually available within 24 hours), detects all genotypes of swine vesicular disease virus, and is sufficiently sensitive for use on samples collected from cases of suspect clinical disease.U.K.
3.Where sub-clinical infection is suspected, or when samples are collected after the resolution of clinical disease or when processing faecal samples, enhanced RT-PCR techniques, like nested RT-PCR, immune-PCR, ELISA-PCR and more elaborate RNA extraction methods produce a detection system at least as sensitive, but considerably more rapid, than multiple passage on tissue culture.U.K.
4.By sequencing approximately 200 nucleotides within the 1D gene which codes for the major structural protein VP1, it is possible to group strains of swine vesicular disease virus according to their sequence homology, and epidemiologically relate strains causing disease in different regions or at different times.U.K.

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