ANNEX IIIStandards for Aujeszky's disease serological tests — Protocol for the enzyme linked immunosorbent assay (ELISA) for detecting antibodies to Aujeszky's disease virus (whole virus), to glycoprotein B (ADV-gB), to glycoprotein D (ADV-gD) or to glycoprotein E (ADV-gE)

2.Standardisation, sensitivity and specificity of the test.

  1. (a)

    The sensitivity of the test must be of such a level that the following Community reference sera are scored positive:

    • Community reference serum ADV 1 at 1:8 dilution,

    • Community reference serum ADV-gE A,

    • Community reference serum ADV-gE B,

    • Community reference serum ADV-gE C,

    • Community reference serum ADV-gE D,

    • Community reference serum ADV-gE E,

    • Community reference serum ADV-gE F.

  2. (b)

    The specificity of the test must be of such a level that the following Community reference sera are scored negative:

    • Community reference serum ADV-gE G,

    • Community reference serum ADV-gE H,

    • Community reference serum ADV-gE J,

    • Community reference serum ADV-gE K,

    • Community reference serum ADV-gE L,

    • Community reference serum ADV-gE M,

    • Community reference serum ADV-gE N,

    • Community reference serum ADV-gE O,

    • Community reference serum ADV-gE P,

    • Community reference serum ADV-gE Q.

  3. (c)

    For batch control, Community reference serum ADV 1 must be scored positive at 1:8 dilution and one of the Community reference serum sera from ADV-gE G to ADV-gE Q, as listed in point (b), must be scored negative.

    For batch control of ADV-gB and ADV-gD kits, Community reference serum ADV 1 must be scored positive at the dilution of 1:2 and Community reference serum Q referred to in (b) should be scored negative.

  4. (d)

    F1The institutes listed below will, in addition, be responsible for checking the quality of the ELISA method in each Member State, and in particular for producing and standardising national reference sera according to the Community reference sera.

    AT

    AGES: Österreichische Agentur für Gesundheit und Ernährungssicherheit GmbH — Institut für veterinärmedizinische Untersuchungen Mödling (Austrian Agency for Health and Consumer Protection-Institute for veterinary investigations Mödling)

    Robert Koch-Gasse 17

    A-2340 Mödling

    Tel.: +43 (0) 505 55-38112

    Fax: +43 (0) 505 55-38108

    E-mail: vetmed.moedling@ages.at

    BE

    CODA — CERVA — VAR

    Veterinary and Agrochemical Research Centre

    Groeselenberg 99

    B-1180 Brussels

    CY

    State Veterinary Laboratory

    Veterinary Services

    1417 Athalassa

    Nicosia

    CZ

    —

    DE

    Friedrich-Loeffler-Institut

    Bundesforschungsinstitut für Tiergesundheit

    Standort Wusterhausen

    Seestraße 55

    16868 Wusterhausen

    Tel.: +49.33979 80-0

    Fax: +49.33979 80-200

    F2DK

    National Veterinary Institute, Technical University of Denmark

    Lindholm

    DK-4771 Kalvehave

    EE

    Veterinaar- ja Toidulaboratoorium

    Kreutzwaldi 30, 51006 Tartu, Estonia

    Tel.: +372 7 386 100

    Faks: + 372 7 386 102

    E-post: info@vetlab.ee

    ES

    Laboratorio Central de Sanidad Animal de Algete

    Carretera de Algete, km 8

    Algete 28110 (Madrid)

    Tel.: +34 916 290 300

    Fax: +34 916 290 598

    E-mail: lcv@mapya.es

    FI

    Finnish Food Safety Authority

    Animal Diseases and Food Safety Research

    Mustialankatu 3

    FI-00790 Helsinki, Finland

    E-mail: info@evira.fi

    Tel: +358 20 772 003 (exchange)

    Fax: +358 20 772 4350

    FR

    Laboratoire d’études et de recherches avicoles, porcines et piscicoles

    AFSSA site de Ploufragan/Brest —

    LERAPP

    BP 53

    22440 Ploufragan

    GB

    Veterinary Laboratories Agency

    New Haw, Addlestone, Weybridge

    Surrey KT15 3NB, UK

    Tel. (44-1932) 341111

    Fax (44-1932) 347046

    GR

    Centre of Athens Veterinary Institutes

    25 Neapoleos Street,

    GR-153 10 Agia Paraskevi Attiki

    Tel.: +30.2106010903

    F2HU

    Mezőgazdasági Szakigazgatási Hivatal Központ, Állat-egészségügyi Diagnosztikai Igazgatóság

    Central Agricultural Office, Veterinary Diagnostic Directorate

    Address: 1149 Budapest, Tábornok u. 2.

    Mailing Address: 1581 Budapest, 146. Pf. 2.

    Tel.: +36 1 460-6300

    Fax: +36 1 252-5177

    E-mail: titkarsag@oai.hu

    IE

    Virology Division

    Central Veterinary Research Laboratory

    Department of Agriculture and Food Laboratories

    Backweston Campus

    Stacumny Lane

    Celbridge

    Co. Kildare

    IT

    Centro di referenza nazionale per la malattia di Aujeszky — Pseudorabbia c/o Istituto zooprofilattico sperimentale della Lombardia e dell'Emilia Romagna,

    Via Bianchi, 9;

    25124 Brescia

    LT

    National Veterinary Laboratory

    (Nacionalinė veterinarijos laboratorija)

    J. Kairiūkščio 10

    LT-08409 Vilnius

    LU

    CODA — CERVA — VAR

    Veterinary and Agrochemical Research Centre

    Groeselenberg 99

    B-1180 Brussels

    LV

    Nacionālais diagnostikas centrs

    (National Diagnostic Centre)

    Lejupes iela 3, Rīga, LV-1076

    Tel.: +371 7620526

    Fax: +371 7620434

    E-mail: ndc@ndc.gov.lv

    MT

    —

    NL

    Centraal Instituut voor DierziekteControle

    CIDC-Lelystad

    Hoofdvestiging: Houtribweg 39

    Nevenvestiging: Edelhertweg 15

    Postbus 2004

    8203 AA Lelystad

    PL

    Laboratory Departement of Swine Diseases

    Państwowy Instytut Weterynaryjny – Państwowy Instytut Badawczy

    Al. Partyzantów 57, 24-100 Puławy

    Tel.: +48.81.886 30 51

    Fax: +48.81.886 25 95

    E-mail: sekretariat@piwet.pulawy.pl

    PT

    Laboratório Nacional de Investigação Veterinária (LNIV)

    Estrada de Benfica, 701

    P-1549-011 Lisboa

    SE

    Statens Veterinärmedicinska Anstalt

    Department of Virology

    SE-751 89 Uppsala

    Tel (46-18) 674000

    Fax (46-18) 674467

    SI

    Univerza v Ljubljani

    Veterinarska fakulteta

    Nacionalni veterinarski inštitut

    Gerbičeva 60,

    SI-1000 Ljubljana

    SK

    Štátny veterinárny ústav,

    Pod dráhami 918,

    960 86 Zvolen