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Commission Decision of 21 June 2005 on the implementation of survey programmes for avian influenza in poultry and wild birds to be carried out in the Member States (notified under document number C(2005) 1827) (Text with EEA relevance) (2005/464/EC)
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D.SURVEY FOR AVIAN INFLUENZA IN WILD BIRDS
In those Member States where surveillance will also concern wild birds the following guidelines shall be followed.
D.1.Survey design and implementation
1.Liaisons with bird conservation/watching institutions and ringing stations are necessary. Sampling where appropriate shall be carried out by staff from these groups/stations or by hunters.
2.Experience with the previous surveys has shown that the virus isolation rate was extremely low, therefore sampling shall focus on the birds migrating south during autumn and early winter.
D.2.Sampling procedures
1.Cloacal swabs for virological examination shall be taken. In addition to ‘first year’ birds in autumn, host species with high susceptibility and increased contact with poultry (such as Mallard ducks) may offer the highest chance of success.
2.Samples shall be taken from different species of free living birds. Waterfowl and shorebirds shall be the main sampling targets.
3.Swabs containing faeces, or carefully collected fresh faeces shall be taken from wild birds trapped, hunted and found freshly dead.
4.Pooling of up to five samples from the same species is possible. Specific care has to be taken for the storage and transport of samples. If rapid transport within 48 hours to the laboratory (in transport medium at 4° Celsius) is not guaranteed, samples shall be stored and then transported in dry ice at – 70° Celsius.
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