Article 2U.K.Product
1.The genetically modified organism to be placed on the market is a carnation (Dianthus caryophyllus L.), with modified flower colour, derived from a Dianthus caryophyllus L. cell culture, and transformed with Agrobacterium tumefaciens, strain AGL0, using the vector pCGP1991, and resulting in line FLO-40685-2.
The genetically modified carnation contains the following DNA in three cassettes:
(a)Cassette 1
The petunia dfr gene encoding dihydroflavonol 4-reductase (DFR), a key enzyme in the anthocyanin biosynthetic pathway, including its own promoter and terminator.
(b)Cassette 2
The promoter sequence from snapdragon chalcone synthase gene, flavonoid 3′5′-hydroxylase (f3′5′h) from Viola hortensis cDNA encoding F3′5′H, a key enzyme in the anthocyanin biosynthetic pathway, and the terminator from the D8 petunia gene encoding a putative phospholipid transfer protein.
These two cassettes were inserted into the plant genome to obtain the desired flower colour.
(c)Cassette 3
The Cauliflower mosaic virus 35S promoter, the 5′-untranslated region from the petunia gene encoding chlorophyll a/b binding protein, the SuRB (als) gene coding for a mutant acetolactate synthase (ALS) derived from Nicotiana tabacum, which confers tolerance to sulfonylurea, including its own terminator. This trait was used as a marker in the selection of transformants.
2.The genetically modified carnation contains the insert, or part of it, in four loci:
Locus 1: one copy of the T-DNA, containing the three cassettes and an incomplete copy of T-DNA containing only the f3′5′h cassette with the right T-DNA border. The two T-DNA copies are separated by a carnation genomic DNA region,
Locus 2: one insert containing the D8 terminator and the right T-DNA border,
Locus 3: one complete and one incomplete copy of the f3′5′h cassette, both containing D8 terminator sequences and the right T-DNA borders in a tail-to-tail orientation,
Locus 4: an incomplete copy of the als cassette and the left T-DNA border.
3.The unique identifier of the genetically modified carnation shall be FLO-4Ø685-2.