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Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)

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Changes over time for: Division 2.1.

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[F1 [F2 [F32.1. Standards U.K.

2.1.1. The Brucella abortus biovar 1 Weybridge strain No 99 or USDA strain 1119-3 must be used for the preparation of all antigens used in the rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and the milk ring test (MRT). U.K.
2.1.2. The standard reference serum for the RBT, SAT, CFT and MRT shall be the OIE international reference standard serum (OIEISS) formerly named WHO second international anti- Brucella abortus Serum (ISAbS). U.K.
2.1.3. The standard reference sera for enzyme-linked immunosorbent assays (ELISAs) shall be: U.K.
  • the OIEISS,

  • the weak positive OIE ELISA standard serum (OIEELISA WP SS),

  • the strong positive OIE ELISA standard serum (OIEELISA SP SS),

  • the negative OIE ELISA standard serum (OIEELISA N SS).

2.1.4. The standard reference sera for fluorescence polarisation assays (FPAs) shall be: U.K.
  • the weak positive OIE ELISA standard serum (OIEELISA WP SS),

  • the strong positive OIE ELISA standard serum (OIEELISA SP SS),

  • the negative OIE ELISA standard serum (OIEELISA N SS).

2.1.5. The standard sera listed in 2.1.3 and 2.1.4 are available from the Community reference laboratory for brucellosis or the Veterinary Laboratories Agency (VLA), Weybridge, United Kingdom. U.K.
2.1.6. The OIEISS, the OIEELISA WP SS, the OIEELISA SP SS and the OIEELISA N SS are international primary standards from which secondary reference national standards serum (working standards) must be established for each test referred to in 2.1.1 in each Member State.] ] ] U.K.

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