Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)

[F1 [F22.1. Standards U.K.

2.1.1. The Brucella abortus biovar 1 Weybridge strain No 99 or USDA strain 1119-3 must be used for the preparation of all antigens used in the rose bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and the milk ring test (MRT). U.K.
2.1.2. The standard reference serum for the RBT, SAT, CFT and MRT is the OIE international reference standard serum (OIEISS) formerly named WHO second international anti- Brucella abortus Serum (ISAbS). U.K.
2.1.3. The standard reference sera for ELISAs are: U.K.
  • the OIEISS,

  • the weak positive OIE ELISA standard serum (OIEELISA WP SS),

  • the strong positive OIE ELISA standard serum (OIEELISA SP SS),

  • The negative OIE ELISA standard serum (OIEELISA N SS).

2.1.4. The above listed standard sera are available from the Veterinary Laboratories Agency (VLA), Weybridge, United Kingdom. U.K.
2.1.5. The OIEISS, the OIEELISA WP SS, the OIEELISA SP SS and the OIEELISA N SS are international primary standards from which secondary reference national standards ( working standards ) must be established for each test in each Member State.] ] U.K.