- Latest available (Revised)
- Point in Time (31/01/2020)
- Original (As adopted by EU)
Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)
You are here:
- Directives originating from the EU
- 1964 No. 432
- ANNEX C
- Division 2.
- Division 2.3.
What Version
Advanced Features
- Show Geographical Extent(e.g. England, Wales, Scotland and Northern Ireland)
- Show Timeline of Changes
More Resources
Revised version PDFs
- Revised 27/05/20151.04 MB
- Revised 01/01/20151.08 MB
- Revised 17/07/20141.20 MB
- Revised 01/07/20131.20 MB
- Revised 18/12/20091.20 MB
- Revised 31/12/20080.43 MB
- Revised 03/09/20080.43 MB
- Revised 13/11/20070.45 MB
- Revised 05/01/20070.45 MB
- Revised 09/07/20050.58 MB
- Revised 01/05/20040.57 MB
- Revised 29/07/20020.59 MB
- Revised 12/04/20020.58 MB
- Revised 31/07/20010.60 MB
- Revised 04/07/20000.68 MB
- Revised 03/05/20002.86 MB
- Revised 31/12/19980.46 MB
- Revised 15/07/19980.46 MB
- Revised 01/07/19980.25 MB
- Revised 30/06/19640.46 MB
Legislation originating from the EU
When the UK left the EU, legislation.gov.uk published EU legislation that had been published by the EU up to IP completion day (31 December 2020 11.00 p.m.). On legislation.gov.uk, these items of legislation are kept up-to-date with any amendments made by the UK since then.
This item of legislation originated from the EU
Legislation.gov.uk publishes the UK version. EUR-Lex publishes the EU version. The EU Exit Web Archive holds a snapshot of EUR-Lex’s version from IP completion day (31 December 2020 11.00 p.m.).
Changes over time for: Division 2.3.
Status:
EU Directives are published on this site to aid cross referencing from UK legislation. Since IP completion day (31 December 2020 11.00 p.m.) no amendments have been applied to this version.
[F1 [F2 [F32.3. Complement fixation test (CFT) U.K.
2.3.1. The antigen represents a bacterial suspension in phenol-saline (NaCl 0,85 % (m/v) and phenol at 0,5 % (v/v)) or in a veronal buffer. Antigens may be delivered in the concentrated state provided the dilution factor to be used is indicated on the bottle label. The antigen must be stored at 4 °C and not frozen. U.K.
2.3.2. Serums must be inactivated as follows: U.K.
bovine serum: 56 to 60 °C for 30 to 50 minutes,
porcine serum: 60 °C for 30 to 50 minutes.
2.3.3. In order to carry out the genuine reaction within the test procedure, a complement dose higher than the minimum necessary for total haemolysis shall be used. U.K.
2.3.4. In carrying out the complement fixation test, the following controls must be made each time: U.K.
(a)
control of the anti-complementary effect of the serum;
(b)
control of the antigen;
(c)
control of sensitised red blood cells;
(d)
control of the complement;
(e)
control using a positive serum of sensitivity at the start of the reaction;
(f)
control of the specificity of the reaction using a negative serum.
2.3.5. Calculation of results U.K.
The OIEISS contains 1 000 international CFT units (ICFTU) per ml. If the OIEISS is tested in a given method the result is given as a titre (i.e. highest direct dilution of the OIEISS giving 50 % haemolysis, T OIEISS ). The test result for the test serum given as titre (T TESTSERUM ) must be expressed in ICFTU per ml. In order to convert the expression of a titre into ICFTU, the factor (F) necessary to convert a titre of an unknown test serum (T TESTSERUM ) tested by that method into the ICFTU expression can be found from the formula:
F = 1 000 × 1/T OIEISS
and the content of international CFT units per ml of test serum (ICFTU TESTSERUM ) from the formula:
ICFTU TESTSERUM = F × T TESTSERUM
2.3.6. Interpretation of results U.K.
A serum containing 20 or more ICFTU per ml shall be considered to be positive.] ] ]
Textual Amendments
F1 Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.
Options/Help
Print Options
PrintThe Whole Directive
PrintThe Whole Annex
PrintThe Whole Division
PrintThis Division only
You have chosen to open the Whole Directive
The Whole Directive you have selected contains over 200 provisions and might take some time to download. You may also experience some issues with your browser, such as an alert box that a script is taking a long time to run.
Would you like to continue?
Legislation is available in different versions:
Latest Available (revised):The latest available updated version of the legislation incorporating changes made by subsequent legislation and applied by our editorial team. Changes we have not yet applied to the text, can be found in the ‘Changes to Legislation’ area.
Original (As adopted by EU): The original version of the legislation as it stood when it was first adopted in the EU. No changes have been applied to the text.
Point in Time: This becomes available after navigating to view revised legislation as it stood at a certain point in time via Advanced Features > Show Timeline of Changes or via a point in time advanced search.
See additional information alongside the content
Geographical Extent: Indicates the geographical area that this provision applies to. For further information see ‘Frequently Asked Questions’.
Show Timeline of Changes: See how this legislation has or could change over time. Turning this feature on will show extra navigation options to go to these specific points in time. Return to the latest available version by using the controls above in the What Version box.
Opening Options
Different options to open legislation in order to view more content on screen at once
More Resources
Access essential accompanying documents and information for this legislation item from this tab. Dependent on the legislation item being viewed this may include:
- the original print PDF of the as adopted version that was used for the EU Official Journal
- lists of changes made by and/or affecting this legislation item
- all formats of all associated documents
- correction slips
- links to related legislation and further information resources
Timeline of Changes
This timeline shows the different versions taken from EUR-Lex before exit day and during the implementation period as well as any subsequent versions created after the implementation period as a result of changes made by UK legislation.
The dates for the EU versions are taken from the document dates on EUR-Lex and may not always coincide with when the changes came into force for the document.
For any versions created after the implementation period as a result of changes made by UK legislation the date will coincide with the earliest date on which the change (e.g an insertion, a repeal or a substitution) that was applied came into force. For further information see our guide to revised legislation on Understanding Legislation.
More Resources
Use this menu to access essential accompanying documents and information for this legislation item. Dependent on the legislation item being viewed this may include:
- the original print PDF of the as adopted version that was used for the print copy
- correction slips
Click 'View More' or select 'More Resources' tab for additional information including:
- lists of changes made by and/or affecting this legislation item
- confers power and blanket amendment details
- all formats of all associated documents
- links to related legislation and further information resources
All content is available under the Open Government Licence v3.0 except where otherwise stated. This site additionally contains content derived from EUR-Lex, reused under the terms of the Commission Decision 2011/833/EU on the reuse of documents from the EU institutions. For more information see the EUR-Lex public statement on re-use.