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Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)
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- Directives originating from the EU
- 1964 No. 432
- ANNEX C
- Division 2.
- Division 2.7.
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Changes over time for: Division 2.7.
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[F1 [F2 [F32.7. Fluorescence polarisation assay (FPA) U.K.
2.7.1. The FPA can be performed in glass tubes or a 96-well plate format. The technique used, its standardisation and the interpretation of results must conform to that specified Chapter 2.4.3 (bovine brucellosis) of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008. U.K.
2.7.2. Standardisation of the test U.K.
The FPA shall be standardised so that:
(a)
the OIEELISA SP SS and OIEELISA WP SS consistently give positive results.
(b)
a 1/8 pre-dilution of the OIEELISA WP SS or a 1/64 pre-dilution of the OIEELISA SP SS made up in a negative serum (or in a negative pool of sera) always gives a negative reaction;
(c)
the OIEELISA N SS always gives a negative reaction.
The following shall be included in each batch of tests: a strong positive, a weak positive, a negative working standard serum (calibrated against the OIE ELISA Standard Sera).] ] ]
Textual Amendments
F1 Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.
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