Print Options
PrintThe Whole
Directive
PrintThe Whole
Annex
PrintThis
Division
only
Changes over time for: Division
3.
Timeline of Changes
This timeline shows the different versions taken from EUR-Lex before exit day and during the implementation period as well as any subsequent versions created after the implementation period as a result of changes made by UK legislation.
The dates for the EU versions are taken from the document dates on EUR-Lex and may not always coincide with when the changes came into force for the document.
For any versions created after the implementation period as a result of changes made by UK legislation the date will coincide with the earliest date on which the change (e.g an insertion, a repeal or a substitution) that was applied came into force. For further information see our guide to revised legislation on Understanding Legislation.
Status:
EU Directives are published on this site to aid cross referencing from UK legislation. Since IP completion day (31 December 2020 11.00 p.m.) no amendments have been applied to this version.
[ [ [3. COMPLEMENTARY TESTS U.K.
3.1. Brucellosis skin test (BST) U.K.
3.1.1. Conditions for the use of BST U.K.
(a) The brucellosis skin test shall not be used for the purpose of certification for intra-Community trade. U.K.
(b) The brucellosis skin test is one of the most specific tests for the detection of brucellosis in unvaccinated animals; however diagnosis must not be made on the basis of positive intradermal reactions alone. U.K.
(c) Bovine animals, tested with negative result in one of the serological tests defined in this Annex and reacting positively to the BST shall be regarded as infected or suspected to be infected. U.K.
(d) Bovine animals, tested with positive result in one of the serological tests defined in this Annex may be subject to a BST in order to support the interpretation of the serological test results; in particular where in officially brucellosis-free or brucellosis-free bovine herds a cross-reaction with antibodies against other bacteria cannot be excluded. U.K.
3.1.2. The test must be carried out by use of a standardised and defined brucellosis allergen preparation that does not contain smooth lipopolysaccharide (LPS) antigen, as this may provoke non-specific inflammatory reactions or interfere with subsequent serological tests. U.K.
The requirements for the production of brucellin shall comply with Section C1 of Chapter 2.4.3 of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008.
3.1.3. Test procedure U.K.
3.1.3.1. A volume of 0.1 ml of brucellosis allergen shall be injected intradermally into the caudal fold, the skin of the flank, or the side of the neck. U.K.
3.1.3.2. The test shall be read after 48- to 72-hours. U.K.
3.1.3.3. The skin thickness at the injection site shall be measured with vernier callipers before injection and at re-examination. U.K.
3.1.3.4. Interpretation of results: U.K.
3.2. Competitive enzyme-linked immunosorbent assay (cELISA) U.K.
3.2.1. Conditions for the use of cELISA U.K.
The cELISA shall not be used for the purpose of certification for intra-Community trade.
Bovine animals, tested with positive result in one of the other serological tests defined in this Annex may be subject to a cELISA in order to support the interpretation of the other serological test results; in particular where in the officially brucellosis-free or brucellosis-free bovine herds a cross-reaction with antibodies against other bacteria cannot be excluded or to eliminate reactions due to residual antibodies produced in response to vaccination with S19.
3.2.2. Test procedure U.K.
The test shall be carried out in accordance with the prescription in Section B(2) of Chapter 2.4.3 of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008.] ] ]
Back to top