F1F2ANNEX D

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Amendments (Textual)

F3CHAPTER IITESTS FOR ENZOOTIC BOVINE LEUKOSIS

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Amendments (Textual)

A.Agar gel immuno-diffusion test for enzootic bovine leukosis

4.The reagents of the tests shall consist of:

  1. (a)

    antigen: the antigen shall contain specific glycoprotein of enzootic bovine leukosis virus which has been standardised against the E05 serum;

  2. (b)

    the test serum;

  3. (c)

    known positive control serum;

  4. (d)

    agar gel:

    • 0,8 % agar,

    • 8,5 % NaCl,

    • 0,05 M Tris-buffer pH 7,2,

    • 15 ml of this agar shall be introduced into a petri dish of 85 mm diameter, resulting in a depth of 2,6 mm of agar.