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a However, pending the adoption of a Directive on the protection of consumers of shellfish products, it is essential that this value be observed in waters in which live shellfish directly edible by man. | |||||||||||||||||||||||
Abbreviations: | |||||||||||||||||||||||
G = guide I = mandatory | |||||||||||||||||||||||
Parameter | G | I | Reference methods of analysis | Minimum sampling and measuring frequency | |||||||||||||||||||
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1. | pH pH unit | 7 — 9 | —ElectrometryMeasured in situ at the time of sampling | Quarterly | |||||||||||||||||||
2. | Temperature oC | A discharge affecting shellfish waters must not cause the temperature of the waters to exceed by more than 2 oC the temperature of waters not so affected | —ThermometryMeasured in situ at the time of sampling | Quarterly | |||||||||||||||||||
3. | Coloration (after filtration) mg Pt/l | [X1A discharge affecting shellfish waters must not cause the colour of the waters after filtration to deviate by more than 100 mg Pt/l from the colour of waters not so affected] | —Filter through a 0·45 µm membranePhotometric method, using the platinum/cobalt scale | Quarterly | |||||||||||||||||||
4. | Suspended solids mg/l | A discharge affecting shellfish waters must not cause the suspended solid content of the waters to exceed by more than 30 % the content of waters not so affected |
| Quarterly | |||||||||||||||||||
5. | Salinity ‰ | 12 to 38 ‰ |
| Conductimetry | Monthly | ||||||||||||||||||
6. | Dissolved oxygen Saturation % | ≥ 80 % |
|
| Monthly, with a minimum of one sample representative of low oxygen conditions on the day of sampling. However, where major daily variations are suspected, a minimum of two samples in one day shall be taken | ||||||||||||||||||
7. | Petroleum hydrocarbons | Hydrocarbons must not be present in the shellfish water in such quantities as to:
| Visual examination | Quarterly | |||||||||||||||||||
8. | Organohalogenated substances | The concentration of each substance in shellfish flesh must be so limited that it contributes, in accordance with Article 1, to the high quality of shellfish products | The concentration of each substance in the shellfish water or in shellfish flesh must not reach or exceed a level which has harmful effects on the shellfish and larvae | Gas chromatograpny after extraction with suitable solvents and purification | Half-yearly | ||||||||||||||||||
9. | Metals
mg/l | The concentration of each substance in shellfish flesh must be so limited that it contributes in accordance with Article 1, to the high quality of shellfish products | The concentration of each substance in the shellfish water or in the shellfish flesh must not exceed a level which gives rise to harmful effects on the shellfish and their larvae The synergic effects of these metals must be taken into consideration | Spectrometry of atomic absorption preceded, where appropriate, by concentration and/or extraction | Half-yearly | ||||||||||||||||||
10. | Faecal coliforms/100 ml | ≤ 300 in the shellfish flesh and intervalvular liquida | Method of dilution with fermentation in liquid substrates in at least three tubes in three dilutions. Subculturing of the positive tubes on a confirmation medium. Count according to MPN (most probable number). Incubation temperature 44 oC ± 0·5 oC | Quarterly | |||||||||||||||||||
11. | Substances affecting the taste of the shellfish | Concentration lower than that liable to impair the taste of the shellfish | Examination of the shellfish by tasting where the presence of one of these substances is presumed | ||||||||||||||||||||
12. | Saxitoxin (produced by dinoflagellates) |