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This method describes the determination of sulphides present in cosmetic products. The presence of thiols or other reducing agents (including sulphites) does not interfere.
The concentration of sulphides determined by this method is expressed as a percentage of sulphur by mass.
After acidification of the medium, hydrogen sulphide is entrained by a stream of nitrogen and then fixed in the form of cadmium sulphide. The latter is filtered and rinsed and then determined by iodometry.
All reagents should be of analytical purity.
5.2. 100 ml round-bottom flask with three standard ground-glass necks.
5.3. Two 150 ml conical flasks with ground-glass necks, fitted with a device comprising a dip tube and a side outlet tube for releasing the entraining gas.
5.4. One long-stem tunnel.
NB: The entraining apparatus must pass the following leak-tightness test: simulating the test conditions, replace the product to be determined by 10 ml of a sulphide solution (prepared from 4.4) containing ‘X mg’ of sulphide (iodometrically determined). Let ‘Y’ be the number of milligrams of sulphide found at the end of this operation. The difference between quantity ‘X’ and quantity ‘Y’ must not exceed 3 %.U.K.
The sulphide content of the sample, expressed as sulphur, as percentage by mass, is calculated by the following formula:
where:
=
the number (in millilitres) of iodine standard solution (4.3) used,
=
the molarity of this solution,
=
the number (in millilitres) of the sodium thiosulphate standard solution (4.2),
=
the molarity of this solution,
=
the mass (in grams) of the test sample.
For a sulphide content of about 2 % (m/m), the difference between the results of two determinations carried out in parallel on the same sample should not exceed an absolute value of 0,2 % (m/m).
Norm ISO 5725.
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