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[F1ANNEX D U.K. AFRICAN HORSE SICKNESS DIAGNOSIS

2. INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST) U.K.

2.2. Interpretation of the results U.K.

2.2.1. Calculate the cut-off value by adding 0,6 to the value of the negative control (0,6 is the standard deviation derived with a group of 30 negative sera). U.K.
2.2.2. Test samples giving absorbance values lower than the cut-off are regarded as negative. U.K.
2.2.3. Test samples giving absorbance values greater than the cut-off + 0,15 are regarded as positive. U.K.
2.2.4. Test samples giving intermediate absorbance values are doubtful and a second technique must be employed to confirm the result.] U.K.