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Council Directive 92/66/EECShow full title
Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease
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- Directives originating from the EU
- 1992 No. 66
- ANNEX III
- CHAPTER 2
- Virus isolation in embryonated...
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- Revised 01/01/20190.30 MB
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- Revised 05/06/20030.13 MB
- Revised 01/01/19950.13 MB
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Changes over time for: Virus isolation in embryonated fowls' eggs
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Virus isolation in embryonated fowls' eggsU.K.
The clarified supernatent fluid should be inoculated in 0,1-0,2 ml amounts into the allantoic cavity of each of a minimum of four embryonated, fowls' eggs which have been incubated for 8 to 10 days. Ideally, these eggs should be obtained from a specific pathogen-free flock, but when this is impracticable it is acceptable to use eggs obtained from a flock shown to be free of antibodies to Newcastle-disease virus. The inoculated eggs are held at 37 °C and candled daily. Eggs with dead or dying embryos as they arise, and all remaining eggs six days after inoculation should be chilled to 4 °C and the allantoic-amniotic fluids tested for haemagglutination activity. If no haemagglutination is detected the above procedure is repeated using undiluted allantoic/amniotic fluid as inoculum.
When haemagglutination is detected the presence of bacteria should be excluded by culture. If bacteria are present the fluids may be passed through a 450 nm membrane filter, further antibiotics added and inoculated into embryonated eggs as above.
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