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Council Directive 92/66/EECShow full title

Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease

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2.Red blood cells (RBC) taken and pooled from a minimum of three specific pathogen free chickens (if not available blood may be taken from birds regularly monitored and shown to be free of NDV antibodies) into an equal volume of Alsever's solution. Cells should be washed three times in PBS before use. For the test a 1 % suspension (packed cell v/v) in PBS is recommended.U.K.

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