Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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Textual Amendments
GENERAL: Unopened sterilized buffers can be stored for up to one year. U.K.
This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
| Na 2 HPO 4 (anhydrous) | 4,26 g |
| KH 2 PO 4 | 2,72 g |
| Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
| Na 2 HPO 4 .12H 2 O | 2,7 g |
| NaH 2 PO 4 .2H 2 O | 0,4 g |
| Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
This buffer is used for dilution of antibodies.
| Na 2 HPO 4 .12H 2 O | 2,7 g |
| NaH 2 PO 4 .2H 2 O | 0,4 g |
| NaCl | 8,0 g |
| Distilled water | 1.0 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
This buffer is used to wash slides.
Add 0,1 % Tween 20 to the IF buffer.
This buffer is used as a mountant fluid on the windows of IF slides to enhance fluorescence.
| Na 2 HPO 4 .12H 2 O | 3,2 g |
| NaH 2 PO 4 .2H 2 O | 0,15 g |
| Glycerol | 50 ml |
| Distilled water | 100 ml |
Anti-fading mountant solutions are commercially available e.g. Vectashield ® (Vector Laboratories) or Citifluor ® (Leica).]
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