Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
| Na 2 HPO 4 (anhydrous) | 4,26 g |
| KH 2 PO 4 | 2,72 g |
| Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
| Na 2 HPO 4 .12H 2 O | 2,7 g |
| NaH 2 PO 4 .2H 2 O | 0,4 g |
| Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.]
Textual Amendments
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