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EU Directives are published on this site to aid cross referencing from UK legislation. Since IP completion day (31 December 2020 11.00 p.m.) no amendments have been applied to this version.
[1. Buffers for extraction procedure U.K.
1.1. Extraction buffer (50 mM phosphate buffer, pH 7,0) U.K.
This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
Na 2 HPO 4 (anhydrous) | 4,26 g |
KH 2 PO 4 | 2,72 g |
Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
|
| Purpose | Quantity (per L) |
---|
Lubrol flakes | Deflocculant | 0,5 g |
DC silicone antifoam | Anti-foam agent | 1,0 ml |
Tetrasodium pyrophosphate | Anti-oxidant | 1,0 g |
Polyvinylpyrrolidone- 40 000 (PVP-40) | Binding of PCR inhibitors | 50 g |
1.2. Pellet buffer (10 mM phosphate buffer, pH 7,2) U.K.
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
Na 2 HPO 4 .12H 2 O | 2,7 g |
NaH 2 PO 4 .2H 2 O | 0,4 g |
Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.]
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