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Council Directive 93/85/EECShow full title
Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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- Revised 07/07/20060.70 MB
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[F11. Buffers for extraction procedure U.K.
1.1. Extraction buffer (50 mM phosphate buffer, pH 7,0) U.K.
This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
| Na 2 HPO 4 (anhydrous) | 4,26 g |
| KH 2 PO 4 | 2,72 g |
| Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
1.2. Pellet buffer (10 mM phosphate buffer, pH 7,2) U.K.
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
| Na 2 HPO 4 .12H 2 O | 2,7 g |
| NaH 2 PO 4 .2H 2 O | 0,4 g |
| Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.]
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