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Textual Amendments
Count the mean number of typical fluorescent cells per field of view (c)
Calculate the number of typical fluorescent cells per microscope slide window (C)
C = c × S/s
where | S | = | surface area of window of multispot slide, and |
s | = | surface area of objective field. |
s = πi 2 /4G 2 K 2 | where | i | = | field coefficient (varies from 8 to 24 depending upon ocular type) |
K | = | tube coefficient (1 or 1,25) | ||
G | = | magnification of objective (100x, 40x etc.). |
Calculate the number of typical fluorescent cells per ml of resuspended pellet (N)
N = C × 1 000 /y × F
where | y | = | volume of resuspended pellet on each window, and |
F | = | dilution factor of resuspended pellet.] |