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Council Directive 93/85/EECShow full title
Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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- Revised 07/07/20060.70 MB
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Version Superseded: 07/07/2006
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— Nitrate reductase activity and denitrification (Bradbury, 1970)U.K.
Culture medium:
| KNO3 (nitrite free) | 1 g |
| Difco bacto yeast extract | 1 g |
| K2HPO4 | 5 g |
| Distilled water | 1 litre |
Dispense into 10 ml volumes in 20 ml bottles. Sterilize by autoclaving at 121 oC for 15 minutes.
Reagent A:
| H2SO4 | 8g |
| 5N acetic acid | 1 litre |
Reagent B:
| naphthylamine | 5 g |
| 5N acetic acid | 1 litre |
Inoculate the nitrate medium in duplicate. Test after 10 and 20 days, by adding one drop of Lugol's iodine, 0,5 ml reagent A and 0,5 ml reagent B. If medium does not turn reddish, add approximately 50 mg zinc powder. Observe the colour reaction.
Positive reaction:
| Colour reaction | ||
|---|---|---|
| Stage 1 | Stage 2 | |
| No reduction of nitrate | colourless | red |
| Reduction of nitrate as far as nitrite (nitrate reductase only) | red | — |
| Reduction of nitrate beyond nitrite (denitrification — nitrate and nitrite reductase) | colourless | colourless |
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