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Version Superseded: 07/07/2006
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EU Directives are published on this site to aid cross referencing from UK legislation. Since IP completion day (31 December 2020 11.00 p.m.) no amendments have been applied to this version.
— Nitrate reductase activity and denitrification (Bradbury, 1970)U.K.
Culture medium:
KNO3 (nitrite free) | 1 g |
Difco bacto yeast extract | 1 g |
K2HPO4 | 5 g |
Distilled water | 1 litre |
Dispense into 10 ml volumes in 20 ml bottles. Sterilize by autoclaving at 121 oC for 15 minutes.
Reagent A:
H2SO4 | 8g |
5N acetic acid | 1 litre |
Reagent B:
naphthylamine | 5 g |
5N acetic acid | 1 litre |
Inoculate the nitrate medium in duplicate. Test after 10 and 20 days, by adding one drop of Lugol's iodine, 0,5 ml reagent A and 0,5 ml reagent B. If medium does not turn reddish, add approximately 50 mg zinc powder. Observe the colour reaction.
Positive reaction:
| Colour reaction |
---|
| Stage 1 | Stage 2 |
---|
No reduction of nitrate | colourless | red |
Reduction of nitrate as far as nitrite (nitrate reductase only) | red | — |
Reduction of nitrate beyond nitrite (denitrification — nitrate and nitrite reductase) | colourless | colourless |
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