Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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The testing procedure is intended for potato tubers and plants with symptoms typical or suspect of ring rot. It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on diagnostic media and, in case of a positive result, identification of the culture as C. m. subsp. sepedonicus .
The testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests, based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as C. m. subsp. sepedonicus . A positive result from only one of the screening tests is not sufficient to consider the sample suspect.
Screening tests and isolation tests must permit a detection threshold of 10 3 to 10 4 cells/ml resuspended pellet, included as positive controls in each series of tests.
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