[F1ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME
Textual Amendments
1. FLOW CHART DIAGRAM PRESENTATION U.K.
1.1. Detection scheme for the diagnosis of Ring Rot in potato tubers and potato plants with symptoms of ring rot U.K.
The testing procedure is intended for potato tubers and plants with symptoms typical or suspect of ring rot. It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on diagnostic media and, in case of a positive result, identification of the culture as C. m. subsp. sepedonicus .
1.2. Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato tubers U.K.
Principle U.K.
The testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests, based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as C. m. subsp. sepedonicus . A positive result from only one of the screening tests is not sufficient to consider the sample suspect.
Screening tests and isolation tests must permit a detection threshold of 10 3 to 10 4 cells/ml resuspended pellet, included as positive controls in each series of tests.
