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Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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This is the original version (as it was originally adopted).
For cultural details, see Appendix 5.
Wilts may also be induced by populations of other bacteria or fungi present in the tuber tissue pellet. These include Erwinia carotovora, subsp. carotovora and E. carotovora subsp. atroseptica, Phoma exigua var. foveata, as well as large populations of saprophytic bacteria. Such wilts can be distinguished from those caused by C. sepedonicum since whole leaves or whole plants wilt rapidly.
Recommendations for further testing are as follows:
excise the stems above the inoculation site and remove the leaves;
macerate the stems in 0,05 M PBS pH 7,0, as in 3.1 to 3.2;
use half of the pellet to perform a Gram stain (4) and an IF test (5);
use the other half to perform a further eggplant test (6) if the Gram stain and/or IF tests are positive. Use a known C. sepedonicum culture and sterile 0,05M PBS controls. If symptoms are not observed in the subsequent test, the sample must be considered negative.
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