F1ANNEX IITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.
Annotations:
Amendments (Textual)
Appendix 2Media for isolation and culture of R. solanacearum
(a)General growth media
Kelman’s Tetrazolium Medium
Casamino acids (Difco) | 1,0 g |
Bacto-Peptone (Difco) | 10,0 g |
Dextrose | 5,0 g |
Bacto-Agar (Difco) | 15,0 g |
Distilled water | 1,0 l |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.
Cool to 50 °C and add a filter-sterilised solution of 2,3,5-triphenyl tetrazolium chloride (Sigma) to obtain a final concentration of 50 mg per l.
Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..