Textual Amendments
Na 2 CO 3 | 6,36 g |
NaHCO 3 | 11,72 g |
Distilled water | 1,00 l |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 minutes.
Sodium sulphite (0.2 %) may be added as antioxidant if required to prevent build up of oxidised aromatic compounds.
NaCL | 80,0 g |
KH 2 PO 4 | 2,0 g |
Na 2 HPO 4 .12H 2 O | 29,0 g |
KCl | 2,0 g |
Distilled water | 1,0 L |
10X PBS | 100 ml |
10 % Tween 20 | 5 ml |
Distilled water | 895 ml |
10X PBS | 10,0 ml |
Polyvinylpyrrolidone-44000 (PVP-44) | 2,0 g |
10 % Tween 20 | 0,5 ml |
Milk powder | 0,5 g |
Distilled water | make up to 100 ml |
Diethanolamine | 97 ml |
Distilled water | 800 ml |
Mix and adjust to pH 9,8 with concentrated HCl.
Make up to 1 L with distilled water.
Add 0,2 g MgCl 2 .
Dissolve 2 phosphatase substrate 5 mg tablets (Sigma) per 15 ml of solution.]