F1ANNEX IITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.
Annotations:
Amendments (Textual)
Appendix 5Determination of contamination level in IF and FISH tests
- 1.
Count the mean number of typical fluorescent cells per field of view (c).
- 2.
Calculate the number of typical fluorescent cells per microscope slide window (C).
C = c × S/s
where
S
=
surface area of window of multispot slide
and
s
=
surface area of objective field
s = πi2/4G2K2
where
i
=
field coefficient (varies from 8 to 24 depending upon ocular type)
K
=
tube coefficient (1 or 1,25)
G
=
magnification of objective (100 ×, 40 × etc.).
- 3.
Calculate the number of typical fluorescent cells per ml of re-suspended pellet (N).
N = C × 1 000/y × F
where
y
=
volume of re-suspended pellet on each window
and
F
=
dilution factor of re-suspended pellet.
Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..