F1ANNEX IITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.

Annotations:

SECTION IAPPLICATION OF THE TEST SCHEME

2.Scheme for detection and identification of Ralstonia solanacearum in samples of asymptomatic potato tubers

Principle:

The testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests (3), based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as R. solanacearum A positive result from only one of the screening tests is not sufficient to consider the sample suspect.

Screening tests and isolation tests must permit detection of 103 to 104 cells/ml of resuspended pellet, included as positive controls in each series of tests.

Image_r00003

Image_r00004