F1ANNEX IITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.
Annotations:
Amendments (Textual)
SECTION VIOPTIMISED PROTOCOLS FOR DETECTION AND IDENTIFICATION OF R. SOLANACEARUM
B.IDENTIFICATION TESTS
2.IF test
2.1.
Prepare a suspension of approximately 106 cells per ml in IF buffer (Appendix 4).
2.2.
Prepare a twofold dilution series of an appropriate antiserum (see website http://forum.europa.eu.int/Public/irc/sanco/Home/main).
2.3.
Apply the IF procedure (Section VI.A.5.).
2.4.
A positive IF test is achieved if the IF titre of the culture is equivalent to that of the positive control.
Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..